Intrinsic and Extrinsic Regulation of Type III Secretion Gene Expression in Pseudomonas Aeruginosa.
Intrinsic and Extrinsic Regulation of Type III Secretion Gene Expression in Pseudomonas Aeruginosa.
复制标题
DOI：
10.3389/fmicb.2011.00089
复制
发表时间：
2011
影响因子：
5.2
通讯作者：
Yahr TL
中科院分区：
文献类型：
--
作者：
Diaz MR;King JM;Yahr TL
铜绿假单胞菌是一种机会性病原体，在医疗保健环境中尤其成问题，它是肺炎、血液和尿路感染的常见原因。铜绿假单胞菌毒力的一个重要决定因素是III型分泌系统（T3SS）。T3SS依赖性中毒是一个复杂的过程，最低限度地需要铜绿假单胞菌与宿主细胞结合，通过宿主细胞膜注射细胞毒性效应蛋白，诱导T3SS基因表达。后一个过程，被称为接触依赖性表达，涉及一个典型的调节级联，激活T3SS基因表达以响应宿主细胞接触。虽然宿主细胞接触是T3SS基因表达的主要激活信号，但多种膜结合调控系统的参与表明，其他环境信号也在控制T3SS的表达中发挥作用。这些调节系统协调T3SS基因表达与许多其他细胞活动，包括运动性、黏液性、多糖生产和生物膜形成。生物体响应的信号尚不清楚，但许多信号似乎与细胞的代谢状态有关，并整合在一个吸收内源性和外源性信息信号的主电路中。在此，我们回顾了解开这种复杂电路的进展，提供了当前知识差距的分析，并强调了未来研究的潜在领域。完全了解控制T3SS基因表达的调控网络将最大限度地为铜绿假单胞菌感染的治疗策略的发展提供机会。
Pseudomonas aeruginosa is an opportunistic pathogen that is particularly problematic in the healthcare setting where it is a frequent cause of pneumonia, bloodstream, and urinary tract infections. An important determinant of P. aeruginosa virulence is a type III secretion system (T3SS). T3SS-dependent intoxication is a complex process that minimally requires binding of P. aeruginosa to host cells, injection of the cytotoxic effector proteins through the host cell plasma membrane, and induction of T3SS gene expression. The latter process, referred to as contact-dependent expression, involves a well-characterized regulatory cascade that activates T3SS gene expression in response to host cell contact. Although host cell contact is a primary activating signal for T3SS gene expression, the involvement of multiple membrane-bound regulatory systems indicates that additional environmental signals also play a role in controlling expression of the T3SS. These regulatory systems coordinate T3SS gene expression with many other cellular activities including motility, mucoidy, polysaccharide production, and biofilm formation. The signals to which the organism responds are poorly understood but many seem to be coupled to the metabolic state of the cell and integrated within a master circuit that assimilates informational signals from endogenous and exogenous sources. Herein we review progress toward unraveling this complex circuitry, provide analysis of the current knowledge gaps, and highlight potential areas for future studies. Complete understanding of the regulatory networks that control T3SS gene expression will maximize opportunities for the development of strategies to treat P. aeruginosa infections.
登录
查看更多内容
影响因子：
4.9
作者：
Gillis, RJ;White, KG;Iglewski, BH
通讯作者：
Iglewski, BH
影响因子：
3.6
作者：
Dasgupta, N;Lykken, GL;Yahr, TL
通讯作者：
Yahr, TL
影响因子：
3.1
作者：
Boucher, JC;Mudd, HYMH;Deretic, V
通讯作者：
Deretic, V
影响因子：
5.4
作者：
Franchi, Luigi;Stoolman, Joshua;Nunez, Gabriel
通讯作者：
Nunez, Gabriel
影响因子：
3.4
作者：
Brannon MK;Davis JM;Mathias JR;Hall CJ;Emerson JC;Crosier PS;Huttenlocher A;Ramakrishnan L;Moskowitz SM
通讯作者：
Moskowitz SM