Ductility and Porosity of Silk Fibroin Films by Blending with Glycerol/Polyethylene Glycol and Adjusting the Drying Temperature.
Ductility and Porosity of Silk Fibroin Films by Blending with Glycerol/Polyethylene Glycol and Adjusting the Drying Temperature.
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DOI：
10.1021/acsbiomaterials.9b01567
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发表时间：
2020-02
影响因子：
5.8
通讯作者：
Yongfeng Wang;Zhaozhu Zheng;Qingqing Cheng;D. Kaplan;Gang Li;Xiaoqin Wang
中科院分区：
文献类型：
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作者：
Yongfeng Wang;Zhaozhu Zheng;Qingqing Cheng;D. Kaplan;Gang Li;Xiaoqin Wang
生物功能膜的延展性和孔隙率是组织工程中机械顺应性和细胞间通讯的关键特性。然而，将这两个关键特性整合到BFF中仍然是一个巨大的挑战。本论文通过与甘油(Gly)和聚乙二醇400(PEG400)的复合以及调节成膜温度来调节蛋白质的自组装过程，从而制备出延展性和孔隙率可调的丝素蛋白(SF)薄膜。典型地，在不同的筛选条件下，当SF/PEG400/Gly的质量比为10：5：3(SPG1053)时，在4℃下制备的BFF具有显著的延展性，其拉伸强度为(2.7±0.2)Mpa，断裂伸长率为(164.24±24.20)%，优于单用SF、SF/Gly或SF/PEG400制备的薄膜，表现出协同增塑作用。此外，在4℃下制备的SPG1053薄膜对荧光标记的右旋糖苷(葡聚糖-TMR)的渗透效率为56.32±0.85%，显著高于在20℃和60℃下制备的薄膜(34.67±3.63%和15.4±1.16%)。延展性好、多孔的SPG1053与人成纤维细胞(HS865.SK)具有良好的细胞相容性。考虑到展示的延展性、分子筛分特性和细胞相容性，这些新的SPG薄膜应该会为细胞培养和组织工程提供新的选择。
Ductility and porosity of biofunctional films (BFFs) are critical properties for mechanical compliance and intercellular communication in tissue engineering. However, it remains a significant challenge to integrate these two key properties into BFFs. Herein, silk fibroin (SF) films with tunable ductility and porosity were prepared by adjusting the protein self-assembly process through combinations with glycerol (Gly) and polyethylene glycol 400 (PEG400) and regulating the film-casting temperature. Typically, among various conditions screened, the BFFs with a mass ratio of SF/PEG400/Gly of 10:5:3 (SPG1053) prepared at 4 °C exhibited remarkable ductility with a tensile strength of 2.7 ± 0.2 MPa and an elongation at break of 164.24 ± 24.20%, superior to films prepared from SF alone, SF/Gly, or SF/PEG400, demonstrating a synergistic plasticizing effect. Furthermore, the SPG1053 films prepared at 4 °C had a permeation efficiency of 56.32 ± 0.85% for fluorescently labeled dextran (dextran-TMR, MW: 10 kDa) after 204 h, significantly higher than films prepared at 20 °C (34.67 ± 3.63%) and 60 °C (15.4 ± 1.16%). Finally, the ductile and porous SPG1053 had excellent cell compatibility with human fibroblasts (Hs 865.SK). Given the demonstrated ductility, molecule-sieving property, and cytocompatibility, these new SPG films should offer new options for cell culture and tissue engineering.