SHEAR-STRESS INDUCED STIMULATION OF MAMMALIAN-CELL METABOLISM
SHEAR-STRESS INDUCED STIMULATION OF MAMMALIAN-CELL METABOLISM
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DOI：
10.1002/bit.260320812
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发表时间：
1988-10-05
影响因子：
3.8
通讯作者：
ESKIN, SG
中科院分区：
文献类型：
--
作者：
FRANGOS, JA;MCINTIRE, LV;ESKIN, SG
开发了一种流动装置，用于研究在良好控制的条件下，锚定依赖性细胞对大范围恒定和脉动剪应力的代谢反应。人脐静脉内皮细胞单层承受高达24dynes/cm2的稳定剪应力，并测定前列环素的产生。流动的开始导致前列环素的产生突然下降到一个长期稳定的速率(SSR)。暴露于流动中的细胞的SSR大于基础释放水平，并且随着剪切力的增加而线性增加。这项研究表明，在一定范围内的切应力可能不会损害哺乳动物细胞的新陈代谢。事实上，在所研究的剪切应力范围内，通过最大化剪切应力来最大化代谢物的产量。
A flow apparatus has been developed for the study of the metabolic response of anchorage‐dependent cells to a wide range of steady and pulsatile shear stresses under well‐controlled conditions. Human umbilical vein endothelial cell monolayers were subjected to steady shear stresses of up to 24 dynes/cm2, and the production of prostacyclin was determined. The onset of flow led to a burst in prostacyclin production which decayed to a long term steady state rate (SSR). The SSR of cells exposed to flow was greater than the basal release level, and increased linearly with increasing shear stress. This study demonstrates that shear stress in certain ranges may not be detrimental to mammalian cell metabolism. In fact, throughout the range of shear stresses studied, metabolite production is maximized by maximizing shear stress.