Ethylene Production, Respiration, & Internal Gas Concentrations in Cantaloupe Fruits at Various Stages of Maturity.

Ethylene Production, Respiration, & Internal Gas Concentrations in Cantaloupe Fruits at Various Stages of Maturity.
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乙烯生产、呼吸、

DOI:
10.1104/pp.37.1.31
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发表时间:
1962
期刊:
影响因子:
7.4
通讯作者:
Harlan K. Pratt
Harlan K. Pratt
中科院分区:
生物学1区
文献类型:
--
作者:
J. M. Lyons;W. Mcglasson;Harlan K. Pratt

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被引文献

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在研究乙烯产生与成熟果实所表现出的呼吸作用的更年期上升之间的关系时,在气相色谱法出现之前,不可能确定乙烯产生的开始时间。这项技术使乙烯浓度达到或低于生理活动阈值的定量测量成为可能。Burg和Thimann(3,4)是第一个将该方法应用于水果成熟的研究;乙烯是用特别灵敏的导热检测器测定的(2)。在最近的工作中,Meigh等人(11,12)使用更灵敏的火焰化探测器,测量到乙烯浓度低至十亿分之一。本研究采用气相色谱法测定了甜瓜果实(Cucumis melo L. var. reticulatis)中乙烯和二氧化碳的演化速率以及乙烯、二氧化碳和氧气的同步内部浓度。乙烯的测定采用高灵敏度的火焰电离检测器,其他气体的分析采用热导检测器。本文报道了两个实验的结果。在第一个实验中,对不同发育阶段收获的哈密瓜的内部大气进行了分析,在第二个实验中进行了分析。在收获的成熟甜瓜从更年期前到更年期成熟的过程中,跟踪了乙烯和二氧化碳的产生速度以及内部大气成分的变化。虽然有一些关于哈密瓜呼吸速率的数值报道(13),但这些数据是第一次呈现出一种呼吸模式,并明确确立了这种水果中更年期的存在。
In studying the relationship between ethylene production and the climacteric rise in respiration exhibited by ripening fruits, it was not possible to determine when ethylene production begins until the advent of gas chromatography. This technique makes available quantitative measurement of ethylene concentrations at or below the threshold of physiological activity. Burg and Thimann (3, 4) were the first to apply the method to studies of fruit ripening; ethylene was determined with an especially sensitive thermal conductivity detector (2). In recent work, Meigh et al. (11, 12), using the more sensitive flameionization detector, measured ethylene concentrations as low as a few parts per billion. Gas chromatography was used in this study of cantaloupe fruits (Cucumis melo L. var. reticulatis) to assess the rate of ethylene and carbon dioxide evolution and concurrent internal concentrations of ethylene, carbon dioxide, and oxygen. For the ethylene determinations, a highly sensitive flame-ionization detector was used, and the other gases were analyzed with a thermal conductivity detector. Results from two experiments are reported. In the first experiment, analyses were performed on the internal atmospheres of cantaloupes harvested at different stages of development, and in the second. changes in the rate of production of ethylene and carbon dioxide and in the composition of the internal atmosphere were followed in harvested mature melons as they progressed from the preclimacteric through the climacteric stages of ripening. While a few values have been reported for the respiratory rate of cantaloupes (13), these data are the first to present a pattern of respiration and establish clearly the presence of the climacteric in this fruit.