Salicylate acutely stimulates 5'-AMP-activated protein kinase and insulin-independent glucose transport in rat skeletal muscles.
Salicylate acutely stimulates 5'-AMP-activated protein kinase and insulin-independent glucose transport in rat skeletal muscles.
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DOI：
10.1016/j.bbrc.2014.09.066
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发表时间：
2014-10
影响因子：
3.1
通讯作者：
Yasuhiro Serizawa;Rieko Oshima;Mitsuki Yoshida;Ichika Sakon;Kazuto Kitani;Ayumi Goto;S. Tsuda;Tatsuya Hayashi
中科院分区：
文献类型：
--
作者：
Yasuhiro Serizawa;Rieko Oshima;Mitsuki Yoshida;Ichika Sakon;Kazuto Kitani;Ayumi Goto;S. Tsuda;Tatsuya Hayashi
水杨酸盐(Salicylate，SAL)最近被认为与人类的降糖效果有关。我们评估了骨骼肌中5‘-AMP激活的蛋白激酶(AMPK)是否参与了SAL对血糖动态平衡的影响。在含有SAL的缓冲液中孵育大鼠快抽动上丘肌和慢抽动比目鱼肌。两种骨骼肌细胞内SAL浓度迅速增加(约5分钟)，AMPKα亚单位的Thr172磷酸化以剂量和时间依赖的方式增加。SAL可增加AMPKα1和AMPKα2的活性。这些酶活性的增加伴随着3-O-甲基-d-葡萄糖转运活性的增加，以及ATP、磷酸肌酸和糖原含量的下降。SAL不改变包括胰岛素受体底物1、Akt和p70核糖体蛋白S6激酶在内的胰岛素受体信号通路的磷酸化。这些结果表明，SAL可能被转运到骨骼肌中，并可能通过剥夺能量来刺激多种肌肉类型的AMPK和葡萄糖的转运。骨骼肌AMPK可能是SAL改善代谢的部分机制。
Salicylate (SAL) has been recently implicated in the antidiabetic effect in humans. We assessed whether 5′-AMP-activated protein kinase (AMPK) in skeletal muscle is involved in the effect of SAL on glucose homeostasis. Rat fast-twitch epitrochlearis and slow-twitch soleus muscles were incubated in buffer containing SAL. Intracellular concentrations of SAL increased rapidly (<5 min) in both skeletal muscles, and the Thr172phosphorylation of the α subunit of AMPK increased in a dose- and time-dependent manner. SAL increased both AMPKα1 and AMPKα2 activities. These increases in enzyme activity were accompanied by an increase in the activity of 3-O-methyl-d-glucose transport, and decreases in ATP, phosphocreatine, and glycogen contents. SAL did not change the phosphorylation of insulin receptor signaling including insulin receptor substrate 1, Akt, and p70 ribosomal protein S6 kinase. These results suggest that SAL may be transported into skeletal muscle and may stimulate AMPK and glucose transport via energy deprivation in multiple muscle types. Skeletal muscle AMPK might be part of the mechanism responsible for the metabolic improvement induced by SAL.