Expression of functional thyroid-stimulating hormone receptor in microglia
Expression of functional thyroid-stimulating hormone receptor in microglia
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小胶质细胞中功能性促甲状腺激素受体的表达
DOI：
10.1016/j.ando.2021.11.009
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Zuojie Luo
中科院分区：
文献类型：
--
作者：
Yuping Liu;Haiyan Yang;Chunfeng Liang;Xuemei Huang;Xiujun Deng;Zuojie Luo
目的：了解促甲状腺激素受体(TSHR)在小胶质细胞中的表达，探讨其功能。材料和方法：采用免疫印迹、免疫细胞荧光和免疫组织化学双重荧光方法检测TSHR在小胶质细胞中的表达。用促甲状腺激素受体刺激性抗体(TSAb)处理小鼠后，检测环腺苷3‘，5’-单磷酸(CAMP)的生成。结果：TSHR蛋白在小鼠小胶质细胞膜上有表达，且主要定位于细胞膜。此外，TSAb还能刺激小鼠小胶质细胞产生cAMP。脑TSHR对TSAb刺激有特异性反应，提示TSHR的表达具有功能性。由于小胶质细胞是维持中枢神经系统环境稳定的先天免疫细胞，在许多神经免疫疾病中起着关键作用，功能性TSHR在小胶质细胞中的表达具有重要的病理生理意义。
Abstract.Objective: The purpose of the present study was to clarify the expression of .thyroid-stimulating hormone receptor (TSHR) in microglial cells, and to explore .its function..Materials and methods: Expression of TSHR in microglia was determined by .Western blot, immunocytofluorescence and double immunohistofluorescence. .Cyclic adenosine 3′,5′-monophosphate (cAMP) production was measured .after thyrotropin receptor stimulating antibody (TSAb) treatment..Results: Results showed that TSHR protein was expressed and mainly located .in the mouse microglia membrane. Moreover, TSAb stimulated cAMP .Page 2 of 17.Journal Pre-proof.production in mouse microglia (p<0.05)..Conclusions: This study demonstrated the presence of TSHR in microglial.cells. Brain TSHR was able to respond specifically to TSAb stimulation, .suggesting that TSHR expression is functional. As microglia are innate immune .cells that maintain environmental stability in the central nervous system and .play a key role in many neuroimmune diseases， expression of functional .TSHR in microglia has important pathophysiological implications.