BLR-1 and BLR-2, key regulatory elements of photoconidiation and mycelial growth in Trichoderma atroviride
BLR-1 and BLR-2, key regulatory elements of photoconidiation and mycelial growth in Trichoderma atroviride
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DOI：
10.1099/mic.0.27346-0
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发表时间：
2004-11-01
期刊：
影响因子：
2.8
通讯作者：
Herrera-Estrella, A
中科院分区：
文献类型：
--
作者：
Casas-Flores, S;Rios-Momberg, M;Herrera-Estrella, A
在真菌中，趋光性、胡萝卜素合成和生殖结构的诱导以及昼夜节律的重置都由蓝光控制。木霉是一种用于生物防治的真菌，在短暂的蓝光脉冲后以同步的方式萌发孢子。由于其明显的简单性，这种反应被选择用于寻求光感受器分离。克隆了两个基因，蓝光调节因子1和2(Blr-1和Blr-2)，分别与粗糙脉孢菌白领1和2相似。BLR-1蛋白具有蓝光感受器的所有特征，而推导的L蛋白的结构表明它与BLR-1通过PAS结构域相互作用形成复合体。相应基因的破坏表明，它们对于蓝光诱导的凝集是必不可少的。Blr-1和Blr-2也被证明是光诱导表达光解酶编码基因(phr-1)所必需的。菌丝体的机械损伤被发现触发了T阿托维利的凝集，这一反应以前没有描述过。这一反应在突变体中没有改变。红光和蓝光对菌丝生长的一种新的影响被发现涉及另一个光感受器，该感受器由BLR蛋白补偿。
In fungi, phototropism, the induction of carotenogenesis and reproductive structures, and resetting of the circadian rhythm are controlled by blue light. Trichoderma atroviride, a fungus used in biological control, sporulates in a synchronized manner following a brief pulse of blue light. Due to its apparent simplicity, this response was chosen for pursuing photoreceptor isolation. Two genes were cloned, blue-light regulators 1 and 2 (blr-1 and blr-2), similar to the Neurospora crassa white-collar 1 and 2, respectively. The BLR-1 protein has all the characteristics of a blue-light photoreceptor, whereas the structure of the deduced l protein suggests that it interacts with BLR-1 through PAS domains to form a complex. Disruption of the corresponding genes demonstrated that they are essential for blue-light-induced conicliation. blr-1 and blr-2 were also shown to be essential for the light-induced expression of the photolyase-encoding gene (phr-1). Mechanical injury of mycelia was found to trigger conicliation of T atroviride, a response not described previously. This response was not altered in the mutants. A novel effect of both red and blue light on mycelial growth was found involving another light receptor, which is compensated by the BLR proteins.