Cytotoxicity, antibacterial and physicochemical properties of a new epoxy resin-based endodontic sealer containing calcium hydroxide
Cytotoxicity, antibacterial and physicochemical properties of a new epoxy resin-based endodontic sealer containing calcium hydroxide
复制标题
含有氢氧化钙的新型环氧树脂基牙髓封闭剂的细胞毒性、抗菌性和理化特性
DOI：
10.4317/jced.56534
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发表时间：
2020
影响因子：
--
通讯作者：
H. Antunes
中科院分区：
文献类型：
--
作者：
Emmanuel João Nogueira Leal Silva;F. Hecksher;V. T. Vieira;R. Vivan;M. Duarte;Sabrina C. Brasil;H. Antunes
本研究评价了一种新型环氧树脂基氢氧化钙封闭剂的细胞毒性、抗菌性能和理化性能。AH Plus用作比较的参考。材料和方法根据ISO-10993-5规范，使用MTT法进行细胞毒性评价，以检查1至4周期间的3 T3细胞活力。使用针对粪肠球菌的直接接触试验评价抗菌活性。根据ISO-6876/2012规范进行不透射线性、溶解度和流动性评价。根据ANSI/ADA-标准-n.57评估凝固时间。在3、24、48、72和168小时测量pH水平。采用t检验对数据进行统计学分析。采用的显著性水平为P<0.05。结果AH Plus和Sealer Plus在操作24 h和1周后显示细胞毒性作用，2周后变为无细胞毒性。两种封闭剂的细胞毒性差异无统计学意义（P>0.05）。直接接触结果显示，两种新鲜制备的封口剂对粪肠球菌具有抗菌作用。然而，7天后，两种密封剂都失去了大部分抗菌效果。尽管AH Plus的不透射线性和血流比Sealer Plus更高（P<0.05），但两种Sealer均显示了最低要求值。两种封闭剂的溶解度无差异（P>0.05）。AH Plus的凝固时间高于Sealer Plus（P<0.05）。AH Plus和Sealer Plus在所有试验期间均显示中性pH值（P<0.05）。结论Sealer Plus具有与AH Plus相当的性能，可用作根管充填剂。关键词：细胞毒性，根管充填材料，根管充填。
Background This study evaluated the cytotoxicity, antibacterial and physicochemical properties of a new epoxy resin-based endodontic sealer containing calcium-hydroxide Sealer Plus. AH Plus was used as a reference for comparison. Material and Methods Cytotoxicity evaluation was performed according to ISO-10993-5 specifications using MTT assay to check the 3T3 cells viability at 1- to 4-week periods. Antibacterial activity was evaluated using a direct contact test against Enterococcus faecalis. Radiopacity, solubility and flow evaluations were performed according to ISO-6876/2012 specifications. Setting time was assessed following the ANSI/ADA-standard-n.57. The pH level was measured at 3,24,48,72, and 168 hours. Data were statistically analyzed using t-test. The significance level adopted was P<0.05. Results AH Plus and Sealer Plus showed cytotoxic effects after 24 hours and 1 week of manipulation and become noncytotoxic after 2 weeks. No differences were observed in the cytotoxicity of both tested sealers (P>0.05). Direct contact results revealed that both freshly prepared sealers has antibacterial effects against Enterococcus faecalis. However, after 7 days both sealers had lost much of their antibacterial effects. Although AH Plus presented higher radiopacity and flow than Sealer Plus (P<0.05), both sealers showed minimum required values. No differences were observed in the solubility of both sealers (P>0.05). AH Plus showed a higher setting time when compared to Sealer Plus (P<0.05). AH Plus and Sealer Plus showed a neutral pH during all tested periods (P<0.05). Conclusions It can be concluded that Sealer Plus showed suitable properties to be used as an endodontic sealer, comparable with those obtained by AH Plus. Key words:Cytotoxicity, root canal filling materials, root canal obturation.