Effect of mutations in the upstream promoter on the transcription of human 5S rRNA genes
Effect of mutations in the upstream promoter on the transcription of human 5S rRNA genes
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DOI：
10.1016/s0167-4781(01)00264-0
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发表时间：
2001-08-30
期刊：
影响因子：
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通讯作者：
Frederiksen, S
中科院分区：
文献类型：
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作者：
Hallenberg, C;Frederiksen, S
人5S rRNA基因有一个12聚体的外部启动子，即D盒，位于编码序列上游约30 bp处。通过定点诱变，制备了58个不同的D盒启动子突变体。虽然D盒中的一些突变允许完全转录，但与真正的基因相比，其他突变将转录活性降低至20-50%，这表明该外部启动子在转录起始中的重要性。从真实基因和D盒突变克隆构建了大量的maxi 5S rRNA基因。用maxi 5S rRNA基因转染HeLa细胞表明，D盒对5S rRNA基因在体内的表达也是重要的。来自不同真核细胞的证据表明，除了内部控制区外，5S rRNA基因的表达还受到外部启动子的调控。(C)2001爱思唯尔科技有限公司。保留所有权利。
The human 5S rRNA gene has a 12-mer external promoter, the D box, localized about 30 bp upstream the coding sequence. By site directed mutagenesis 58 different D box promoter mutants were made. While some mutations in the D box allowed full transcription, other mutations decreased the transcriptional activity to 20-50% compared to the bona fide gene, showing the importance of this external promoter in transcription initiation. A number of maxi 5S rRNA genes were constructed from bona fide genes and D box mutated clones. Transfection of HeLa cells with maxi 5S rRNA genes showed that the D box is also important for 5S rRNA gene expression in vivo. Evidence from different eukaryotic cells suggests that expression of 5S rRNA genes is regulated by external promoters in addition to the internal control region. (C) 2001 Elsevier Science B.V. All rights reserved.