RADIOIMMUNOASSAY MEASUREMENT OF PROSTAGLANDINS-E, A, AND F IN HUMAN PLASMA
RADIOIMMUNOASSAY MEASUREMENT OF PROSTAGLANDINS-E, A, AND F IN HUMAN PLASMA
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DOI：
10.1172/jci107196
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发表时间：
1973-01-01
影响因子：
15.9
通讯作者：
PARKER, CW
中科院分区：
文献类型：
--
作者：
JAFFE, BM;BEHRMAN, HR;PARKER, CW
描述了能够测量人和大鼠血浆中5 pg前列腺素A、E和F（PGA、PGE和PGF）的放射免疫测定的详细信息。在表观pH值为5.8的有机溶剂系统中提取血浆样品（加入4000 cpm [3 H] PGE 1以计算回收率），然后在草酸柱上进行色谱分析，用浓度递增的甲醇分离PGA、PGE和PGF。使用同源抗体和氚标记物，通过放射免疫测定法测量每个色谱样品。40名正常人的PGA、PGE和PGF的平均血浆浓度分别为1062±107 pg/ml、385±30 pg/ml和141±15 pg/ml。在甲状腺髓样癌、类癌和神经母细胞瘤患者的血浆中检测到PGE水平升高。用吲哚美辛治疗大鼠，使血清PGE水平降低67%。放射免疫分析法似乎是相当大的实验以及临床利益。
The details of a radioimmunoassay capable of measuring as 5 pg of prostaglandin A, E, and F (PGA, PGE, and PGF) in human and rat plasma are described. Plasma samples are extracted (with 4000 cpm [3H] PGE1added for calculation of recovery) with an organic solvent system at an apparent pH of 5.8 and then chromatographed on silicic acid columns with increasing concentrations of methanol to separate PGA, PGE, and PGF. Each chromatographed sample is measured by radioimmunoassay, using the homologous antibody and tritiated marker. 40 normal individuals had mean plasma concentrations of PGA, PGE, and PGF of 1062±107 pg/ml, 385±30 pg/ml, and 141±15 pg/ml, respectively. Elevated PGE levels were measured in the plasma of patients with medullary carcinoma of the thyroid, carcinoid, and neuroblastoma. Treatment of rats with indomethacin decreased serum PGE levels by 67%. The radioimmunoassay appears to be of considerable experimental as well as clinical interest.