Prox 1 in eye degeneration and sensory organ compensation during development and evolution of the cavefish Astyanax
Prox 1 in eye degeneration and sensory organ compensation during development and evolution of the cavefish Astyanax
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DOI：
10.1007/s004270050308
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发表时间：
2000-05-01
影响因子：
2.4
通讯作者：
Tomarev, SI
中科院分区：
文献类型：
--
作者：
Jeffery, WR;Strickler, AG;Tomarev, SI
我们研究了穴居鱼胚胎眼睛退化和感觉器官补偿过程中同源框基因 Prox 1 的表达。墨西哥硬骨鱼 Astyanax mexicans 由有视力的表层鱼类（表层鱼）和数个盲眼洞穴鱼类（洞穴鱼）组成，它们都是独立进化的。眼睛的形成是在穴居鱼发育过程中开始的，但晶状体囊泡经历细胞凋亡，眼睛随后出现并退化。 Prox 1 对小鼠晶状体纤维分化和 γ-晶状体蛋白表达的要求表明，该基因表达的变化可能与穴居鱼眼睛变性有关，用 Prox 1 抗体染色的表层鱼和穴居鱼胚胎显示，Prox 1 在晶状体、神经视网膜、肌节、心脏、后脑和肠道中表达，正如在其他脊椎动物中报道的那样。我们发现 Prox 1 表达在洞穴鱼晶状体发育过程中没有改变。 Prox 1 蛋白在每个洞穴鱼种群的晶状体囊泡形成后就被检测到，并一直持续到晶状体退化时。洞穴鱼晶状体囊泡也被证明表达 γ-晶状体蛋白基因，这表明 Prox 1 在洞穴鱼晶状体发育中发挥功能。除了上述组织外，Prox 1还在洞穴鱼发育中的味蕾和神经丘中表达，这些神经丘的表达得到增强以补偿失明。结论是，Prox 1 基因不参与晶状体变性，但 Prox 1 表达域的扩展发生在穴居鱼味蕾和神经丘发育过程中。
We have investigated expression of the homeobox gene Prox 1 during eye degeneration and sensory organ compensation in cavefish embryos. The teleost Astyanax mexicans consists of sighted surface-dwelling forms (surface fish) and several populations of blind cave-dwelling forms (cavefish), which have evolved independently. Eye formation is initiated during cavefish development, but the lens vesicle undergoes apoptosis, and the eye subsequently attests and degenerates. The requirement of Prox 1 for lens fiber differentiation and gamma-crystallin expression in the mouse suggests that changes in the expression of this gene could be involved in cavefish eye degeneration, Surface fish and cavefish embryos stained with a Prox 1 antibody showed Prox 1 expression in the lens, neuroretina, myotomes, heart, hindbrain, and gut, as reported in other vertebrates. We found that Prox 1 expression is not altered during cavefish lens development. Prox 1 protein was detected in the lens vesicle as soon as it formed and persisted until the time of lens degeneration in each cavefish population. The cavefish lens vesicle was also shown to express a gamma-crystallin gene, suggesting that Prox 1 is functional in cavefish lens development. In addition to the tissues described above, Prox 1 is expressed in developing taste buds and neuromasts in cavefish, which are enhanced to compensate for blindness. It is concluded that the Prox 1 gene is not involved in lens degeneration, but that expansion of the Prox 1 expression domain occurs during taste bud and neuromast development in cavefish.