The tmRNA ribosome-rescue system.
The tmRNA ribosome-rescue system.
复制标题
DOI：
10.1016/b978-0-12-386497-0.00005-0
复制
发表时间：
2012
影响因子：
--
通讯作者：
Hayes, Christopher S.
中科院分区：
文献类型：
--
作者：
Janssen, Brian D.;Hayes, Christopher S.
细菌 tmRNA 质量控制系统监测蛋白质合成并在称为“核糖体救援”的过程中回收停滞的翻译复合物。在救援过程中，tmRNA 首先作为转移 RNA 结合停滞的核糖体，然后作为信使 RNA 将 ssrA 肽标签添加到新生多肽链的 C 末端。 ssrA 肽针对标记肽进行蛋白水解，确保快速降解潜在有害的截短多肽。核糖体救援还促进负责翻译停滞的受损信息的周转。因此，tmRNA 通过促进全长蛋白质的合成来增加基因表达的保真度。除了作为全球质量控制系统外，tmRNA 还在细菌发育、发病机制和环境应激反应中发挥着重要作用。本综述重点关注 tmRNA 介导的核糖体拯救机制以及 tmRNA 在细菌生理学中的作用。
The bacterial tmRNA quality control system monitors protein synthesis and recycles stalled translation complexes in a process termed “ribosome rescue”. During rescue, tmRNA acts first as a transfer RNA to bind stalled ribosomes, then as a messenger RNA to add the ssrA peptide tag to the C-terminus of the nascent polypeptide chain. The ssrA peptide targets tagged peptides for proteolysis, ensuring rapid degradation of potentially deleterious truncated polypeptides. Ribosome rescue also facilitates turnover of the damaged messages responsible for translational arrest. Thus, tmRNA increases the fidelity of gene expression by promoting the synthesis of full-length proteins. In addition to serving as a global quality control system, tmRNA also plays important roles in bacterial development, pathogenesis and environmental stress responses. This review focuses on the mechanism of tmRNA-mediated ribosome rescue and the role of tmRNA in bacterial physiology.