Genes involved in the repression of mutacin I production in Streptococcus mutans.
Genes involved in the repression of mutacin I production in Streptococcus mutans.
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DOI：
10.1099/mic.0.021303-0
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发表时间：
2009-02
期刊：
影响因子：
--
通讯作者：
Qi F
中科院分区：
文献类型：
--
作者：
Nguyen T;Zhang Z;Huang IH;Wu C;Merritt J;Shi W;Qi F
变形链球菌被认为是人类龋病的主要病原体。它能够产生一种名为MUTACINS的多肽抗生素，这可能在它在牙齿生物膜中的入侵和建立中发挥重要作用。变形链球菌UA140产生两种类型的突变素，一种是抗生素突变素I，另一种是非长链霉菌素IV。在先前的研究中，我们构建了一个随机插入-突变文库来筛选参与调节突变素I产生的基因，发现了25个对突变素I产生有积极影响的基因/操纵子。在这项研究中，我们继续我们之前的工作，以确定与突变蛋白I产生负相关的基因。通过使用抑制野生型突变素I产生的高磷BHI平板，我们分离到77个在抑制条件下持续产生突变素I的克隆。从我们能够获得的34个克隆中，我们鉴定了17个独特的基因。这些基因包含多种功能基团，包括中心代谢、表面结合、糖运输和未知功能。17个突变中的一些被进一步鉴定，并显示在生长过程中增加了突变蛋白基因的表达，而突变蛋白通常在野生型中不表达。这些结果进一步证明了突变蛋白的产生和整体细胞内稳态之间的密切而复杂的联系。
Streptococcus mutans is considered a primary pathogen for human dental caries. Its ability to produce a variety of peptide antibiotics called mutacins may play an important role in its invasion and establishment in the dental biofilm. S. mutans strain UA140 produces two types of mutacins, the lantibiotic mutacin I and the non-lantibitoc mutacin IV. In a previous study, we constructed a random insertional-mutation library to screen for genes involved in regulating mutacin I production, and found 25 genes/operons that have a positive effect on mutacin I production. In this study, we continued our previous work to identify genes that are negatively involved in mutacin I production. By using a high phosphate BHI plate that inhibited mutacin I production of the wild-type, we isolated 77 clones that consistently produced mutacin I under repressive conditions. From the 34 clones that we were able to obtain a sequence, 17 unique genes were identified. These genes encompass a variety of functional groups including the central metabolism, surface binding, sugar transport, and unknown functions. Some of the 17 mutations were further characterized and shown to increase mutacin gene expression during growth when it is usually not expressed in the wild-type. These results further demonstrate an intimate and intricate connection between mutacin production and the overall cellular homeostasis.
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