Surface Area to Volume Ratio of Electrospun Polydioxanone Templates Regulates the Adsorption of Soluble Proteins from Human Serum
Surface Area to Volume Ratio of Electrospun Polydioxanone Templates Regulates the Adsorption of Soluble Proteins from Human Serum
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DOI：
10.3390/bioengineering6030078
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发表时间：
2019-09-01
影响因子：
4.6
通讯作者：
Bowlin, Gary L.
中科院分区：
文献类型：
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作者：
Fetz, Allison E.;Fantaziu, Cristina A.;Bowlin, Gary L.
中性粒细胞是第一个与生物材料表面吸附蛋白相互作用的细胞，已越来越多地被认为是引导组织再生的异物反应中的关键大师。最近的研究表明，电纺组织再生模板的小直径（SD）纤维具有较高的表面积与体积比（SAVR），与大直径（LD）纤维相比，可增强中性粒细胞胞外陷阱（NET）的释放，从而导致组织再生受损。在这项研究中，我们评估了八种人血清蛋白在电纺模板表面的吸附，以研究蛋白质吸附如何调节 NET 的释放。由具有高 SAVR 的 SD 纤维和具有低 SAVR 的 LD 纤维制成的电纺聚二氧环己酮模板与 0.2% 人血清一起孵育，并通过基于红外的免疫检测对原位蛋白质吸附进行定量。在检测到的蛋白质中，IgM 和玻连蛋白的吸附水平较低，表明它们在 NET 的释放中不起核心作用。相比之下，白蛋白和 IgG 快速吸附到模板表面。与白蛋白相比，模板上吸附的 IgG 多 100 至 200 倍，并且在具有高 SAVR 的 SD 模板上发生的吸附明显更大。鉴于中性粒细胞表达在吞噬作用和 NET 释放过程中与 IgG 相互作用的受体，这些结果表明 IgG 在 SD 电纺模板上的 SAVR 依赖性吸附可能有助于 NET 释放的上调。总体而言，这项研究可能有助于设计调节 NET 释放的免疫调节生物材料，从而有助于中性粒细胞驱动的组织再生的潜力。
Neutrophils, the first cells that interact with surface-adsorbed proteins on biomaterials, have been increasingly recognized as critical maestros in the foreign body response for guided tissue regeneration. Recent research has shown that small diameter (SD) fibers of electrospun tissue regeneration templates, which have a high surface area to volume ratio (SAVR), enhance the release of neutrophil extracellular traps (NETs) compared to large diameter (LD) fibers, resulting in impaired tissue regeneration. In this study, we evaluated the adsorption of eight human serum proteins on the surface of electrospun templates to investigate how protein adsorption may regulate the release of NETs. Electrospun polydioxanone templates made from SD fibers with high SAVR and LD fibers with low SAVR, were incubated with 0.2% human serum and in situ protein adsorption was quantified with infrared-based immunodetection. Of the detected proteins, IgM and vitronectin adsorbed at low levels, suggesting that they do not play a central role in the release of NETs. Contrastingly, albumin and IgG adsorbed rapidly to the surface of the templates. One-hundred to 200 times more IgG adsorbed on the templates compared to albumin, with significantly greater adsorption occurring on the SD templates with high SAVR. Given that neutrophils express receptors that interact with IgG during phagocytosis and NET release, these results suggest that SAVR-dependent adsorption of IgG on the SD electrospun templates may contribute to the up-regulated release of NETs. Overall, this study may aid in the design of immunomodulatory biomaterials that regulate NET release and thus the potential for neutrophil-driven tissue regeneration.