Expression of Drosophila neoplastic tumor suppressor genes discslarge, scribble, and lethal giant larvae in the mammalian ovary
Expression of Drosophila neoplastic tumor suppressor genes discslarge, scribble, and lethal giant larvae in the mammalian ovary
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DOI：
10.1016/s1567-133x(02)00096-0
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发表时间：
2003-03-01
影响因子：
1.2
通讯作者：
Goode, S
中科院分区：
文献类型：
--
作者：
Huang, JHY;Rajkovic, A;Goode, S
调节无脊椎动物和哺乳动物卵泡发生的分子机制的相似性和差异正开始被解读。在果蝇中，肿瘤抑制基因disslarge对于抑制卵泡细胞相对于生长中的卵母细胞的增殖和运动至关重要。致命的巨型幼虫和涂鸦扮演着相似的角色，并被建议与disslarge密切合作。我们已经鉴定并确定了这些果蝇基因的小鼠同源物的表达模式。原位数据显示，小鼠discslarge-1、discslarge-3、discslarge-4、致命巨型幼虫和scribble在成熟卵泡的卵母细胞和颗粒细胞中以重叠和不同的模式表达。 Disclarge-4 在表面上皮细胞中表达，并在小鼠致癌表面上皮细胞中丢失。所有这些基因以及discslarge-2 和discslarge-5 均在人类卵巢中表达。我们的数据表明，与果蝇一样，这些肿瘤抑制因子可能在哺乳动物卵泡发生过程中合作，但也具有不同的功能。 (C) 2003 Elsevier Science B.V. 保留所有权利。
The similarities and differences in molecular mechanisms regulating invertebrate and mammalian folliculogenesis are starting to be deciphered. In Drosophila, the neoplastic tumor suppressor gene discslarge is crucial for suppressing proliferation and movement of follicle cells relative to the growing oocyte. Lethal giant larvae and scribble play similar roles and have been suggested to collaborate intimately with discslarge. We have identified and determined the expression pattern of murine homologs of these Drosophila genes. In situ data shows that murine discslarge-1, discslarge-3, discslarge-4, lethal giant larvae, and scribble are expressed in both overlapping and distinct patterns in oocytes and granulosa cells in maturing follicles. Disclarge-4 is expressed in the surface epithelium and is lost in mouse carcinogenic surface epithelial cells. All of these genes, as well as discslarge-2 and discslarge-5, are expressed in human ovaries. Our data suggests that as in Drosophila, these tumor suppressors may cooperate during mammalian folliculogenesis, but also have distinct functions. (C) 2003 Elsevier Science B.V. All rights reserved.