3 CLONAL TYPES OF KERATINOCYTE WITH DIFFERENT CAPACITIES FOR MULTIPLICATION
3 CLONAL TYPES OF KERATINOCYTE WITH DIFFERENT CAPACITIES FOR MULTIPLICATION
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DOI：
10.1073/pnas.84.8.2302
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发表时间：
1987-04-01
影响因子：
11.1
通讯作者：
GREEN, H
中科院分区：
文献类型：
--
作者：
BARRANDON, Y;GREEN, H
集落形成的人表皮细胞在其持续生长的能力上是异质的。一旦从单个细胞衍生出克隆，就可以从单次平板接种产生的殖民地类型来估计其生长潜力，并且可以将克隆分配到三个类别中的一个。全无性系具有最大的繁殖能力：在标准条件下，全无性系细胞形成的菌落中只有不到5%的菌落会流产并最终分化。副克隆只包含具有短复制寿命（不超过15代细胞）的细胞，之后它们均匀地中止并最终分化。第三种类型的克隆，部分克隆，包含不同生长潜力的细胞的混合物，是全克隆和副克隆之间的过渡阶段。不同克隆类型的发生率受衰老的影响，因为来自老年供体表皮的细胞产生较低比例的全克隆和较高比例的副克隆。
Colony-forming human epidermal cells are heterogeneous in their capacity for sustained growth. Once a clone has been derived from a single cell, its growth potential can be estimated from the colony types resulting from a single plating, and the clone can be assigned to one of three classes. The holoclone has the greatest reproductive capacity: under standard conditions, fewer than 5% of the colonies formed by the cells of a holoclone abort and terminally differentiate. The paraclone contains exclusively cells with a short replicative lifespan (not more than 15 cell generation), after which they uniformly abort and terminally differentiate. The third type of clone, the meroclone, contains a mixture of cells of different growth potential and is a transitional stage between the holoclone and the paraclone. The incidence of the different clonal types is affected by aging, since cells originating from the epidermis of older donors give rise to a lower proportion of holoclones and a higher proportion of paraclones.