Acute phase protein response in goats
Acute phase protein response in goats
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DOI：
10.1177/104063870802000507
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发表时间：
2008-09-01
影响因子：
1.5
通讯作者：
Ceron, Jose J.
中科院分区：
文献类型：
--
作者：
Gonzalez, Felix H. D.;Tecles, Fernando;Ceron, Jose J.
急性时相蛋白（APPs）是动物炎症紊乱的重要诊断指标。本研究的目的是验证分析方法测量结合珠蛋白（Hp），血清淀粉样蛋白A（SAA），酸溶性糖蛋白（ASG），纤维蛋白原，白蛋白浓度在山羊和评估他们的反应，在这个物种的炎症刺激。所有APP的批内和批间变异系数（CV）分别在0.07-9.31%和1.83- 12.68%范围内，显示出良好的精密度。除纤维蛋白原（通过热沉淀法测定）外，所有测定均以线性方式在不同样品稀释度下测定APP，相关系数较高。皮下注射松节油可引起血清Hp、SAA、ASG和纤维蛋白原浓度的升高和白蛋白浓度的降低。
Acute phase proteins (APPs) are important diagnostic indicators of inflammatory disturbances in animals. The objectives of the current study were to validate analytical methods for measuring haptoglobin (Hp), serum amyloid A (SAA), acid soluble glycoprotein (ASG), fibrinogen, and albumin concentrations in goats and to evaluate their response to an inflammatory stimulus in this species. Intra- and interassay coefficients of variation (CVs) were in the range 0.07-9.31% and 1.83-12.68%, respectively, for all APPs and showed good precision. All assays determined APPs in a linear manner at different sample dilutions with high correlation coefficients with the exception of fibrinogen, which was measured by the heat precipitation method. Subcutaneous injection Of turpentine oil induced all increase in Hp, SAA, ASG, and fibrinogen serum concentrations and a decrease in albumin concentration.