Importance of thymidine kinase activity for normal growth of lumpy skin disease virus (SA-Neethling) - Brief report
Importance of thymidine kinase activity for normal growth of lumpy skin disease virus (SA-Neethling) - Brief report
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DOI：
10.1007/s007050200016
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发表时间：
2002-01-01
影响因子：
2.7
通讯作者：
Viljoen, GJ
中科院分区：
文献类型：
--
作者：
Wallace, DB;Viljoen, GJ
为了研究完整胸苷激酶（TK）基因对南部非洲capripoxvirus疫苗株即瘤状皮肤疾病病毒（LSDV） （SA-Neethling型）的重要性，构建了表达大肠杆菌对半乳糖苷酶（lacZ）报告基因的TK破坏重组蛋白。对重组和野生型（wt） LSDV在tk阳性原代、继代细胞和tk阴性继代细胞中的生长情况进行了比较研究。结果发现，虽然重组病毒和wt病毒在没有选择的TK阳性细胞中都能生长，但重组病毒无法在TK阴性细胞中生长（有或没有选择），这表明TK活性对LSDV的正常生长很重要，即使不是必需的。
In order to study the importance of an intact thymidine kinase (TK) gene for the vaccine strain of a southern African capripoxvirus, namely, lumpy skin disease virus (LSDV) (type SA-Neethling), a TK disruption recombinant was generated expressing the Escherichia coli p-galactosidase (lacZ) reporter gene. A comparative growth study of the recombinant and wild-type (wt) LSDV in TK-positive primary and secondary cells and TK-negative secondary cells was performed. It was found that although recombinant and wt virus both grew in TK-positive cells without selection, the recombinant was unable to grow in TK-negative cells (with or without selection), indicating that TK activity is important, if not essential, for normal growth of LSDV.