Multiresidue determination of (fluoro)quinolone antibiotics in chicken by polymer monolith microextraction and field-amplified sample stacking procedures coupled to CE-UV.
Multiresidue determination of (fluoro)quinolone antibiotics in chicken by polymer monolith microextraction and field-amplified sample stacking procedures coupled to CE-UV.
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DOI：
10.1016/j.talanta.2010.07.055
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发表时间：
2010-09
期刊：
影响因子：
6.1
通讯作者：
Haibo He;Xiaobao Lv;Qiongwei Yu;Yuqi Feng
中科院分区：
文献类型：
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作者：
Haibo He;Xiaobao Lv;Qiongwei Yu;Yuqi Feng
采用基于聚甲基丙烯酸-乙二醇二甲基丙烯酸酯（MAA-EGDMA）整体柱微萃取（PMME）的毛细管电泳-紫外（CE-UV）联用场放大样品堆积（FASS）在线富集技术，实现了9种氟喹诺酮类抗生素（FQs）的同时测定。系统地研究了样品溶液中酸和有机溶剂的组成、进样时间和电压对样品堆积效率的影响。优化了影响PMME萃取效率的几个参数，如样品溶液的pH值、萃取体积、洗涤和解吸条件。在所提出的方法中，通过在CE分析之前将PMME程序与在线预浓缩FASS相结合，实现了所有测试的FQs的灵敏度的大幅增加。9种荧光定量因子的线性关系良好，相关系数（R）均在0.9954以上。方法的检出限（S/N=3）为2.4-34.0ngg-1，回收率为81.2 - 100%，相对标准偏差小于11.3%。建立了PMME-FASS-CE法测定鸡肉中氟喹诺酮类药物残留量的方法。
Simultaneous determination of 9 (fluoro)quinolone antibiotics (FQs) was accomplished by capillary electrophoresis-ultraviolet (CE-UV) based on poly(methacrylic acid-co-ethylene glycol dimethacrylate) (MAA-EGDMA) monolith microextraction (PMME) coupled with on-line preconcentration technique of field-amplified sample stacking (FASS). The effects of composition of the acid and organic solvent in the sample solution, sampling time, and voltage on the efficiency of the sample stacking have been systematically investigated. Several parameters that influence extraction efficiency for PMME such as pH of sample solution, extraction volume, and wash and desorption conditions were optimized. In the proposed method, a substantial increase in sensitivity for all the FQs tested was achieved by the combination of PMME procedure with on-line preconcentration of FASS prior to CE analysis. Good linearities were obtained for the 9 tested FQs with the correlation coefficients (R) above 0.9954. The limits of detection (S/N=3) were found to be 2.4–34.0ngg−1and the recoveries ranged from 81.2 to 100% with relative standard deviations less than 11.3%. The proposed PMME–FASS–CE method was applied to the determination of FQs residues in chicken samples.