Essential role of the thymus to reconstitute naive (CD45RA(+)) T-helper cells after human allogeneic bone marrow transplantation
Essential role of the thymus to reconstitute naive (CD45RA(+)) T-helper cells after human allogeneic bone marrow transplantation
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DOI：
10.1182/blood.v90.2.850.850_850_857
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发表时间：
1997-07-15
期刊：
影响因子：
20.3
通讯作者：
Fink, FM
中科院分区：
文献类型：
--
作者：
Heitger, A;Neu, N;Fink, FM
为了有助于理解人类胸腺在骨髓移植（BMT）后幼稚（CD 45 RA（+））T细胞重建中的作用，我们比较了一种独特情况下的T细胞再生，即胸腺切除的癌症患者（15岁），与同种异体BMT后胸腺携带患者。这些病例具有移植的共同特征（全身照射，HLA匹配的供体，以及用环孢素A预防移植物抗宿主病），并且都具有简单的移植后病程。如荧光激活细胞分选分析所示，胸腺切除的宿主甚至在BMT后24个月也未能重建CD 45 RA（+）T辅助细胞（CD 4（+）细胞中的11%CD45RA（+））。在该患者中，CD 45 RO（+）细胞优先促进CD 4（+）细胞的恢复（骨髓移植后6个月时206/261/μ L，24个月时463/558/μ L，CD 4（+）细胞中的CD 45 RA（+）），而CD 45 RA（+）细胞仍然较低（
To contribute to the understanding of the role of the thymus in humans in the reconstitution of naive (CD45RA(+)) T cells after bone marrow transplantation (BMT), we compared T-cell regeneration in a unique situation, namely a thymectomized cancer patient (15 years old), with that of thymus-bearing patients after allogeneic BMT. These cases shared features of transplantation (total body irradiation, HLA-matched donors, and graft-versus-host disease prophylaxis with cyclosporine A) and all had an uncomplicated posttransplantation course. As shown by fluorescence-activated cell sorting analyses, the thymectomized host failed to reconstitute CD45RA(+) T-helper cells even 24 months after BMT (11% CD45RA(+) of CD4(+) cells). In this patient, preferentially CD45RO(+) cells contributed to the recovery of CD4(+) cells (206 of 261/mu L at 6 months and 463 of 558/mu L at 24 months after BMT, CD45RA(+) of CD4(+) cells), whereas CD45RA(+) cells remained low (