Strategies for the genetic modification of the medicinal plant Catharanthus roseus (L.) G. Don
Strategies for the genetic modification of the medicinal plant Catharanthus roseus (L.) G. Don
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DOI：
10.1007/s11101-006-9020-6
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发表时间：
2007-03
影响因子：
7.7
通讯作者：
R. Zárate;R. Verpoorte
中科院分区：
文献类型：
--
作者：
R. Zárate;R. Verpoorte
这篇综述描述了不同的植物转化技术，包括根癌农杆菌和农杆菌的引导感染。发根、粒子轰击和原生质体融合，已尝试创建转基因长春花（L.）G.Don细胞培养物、毛状根和整个植物。该综述还重点关注用于操纵和提高各种培养系统中次生代谢产物产量的不同方法，特别关注所取得的最相关的结果。最后，从未来的角度来看，作者提出了几种可能在该物种中实施的方法，以尝试促进抗肿瘤药物长春花碱和长春新碱的积累。还给出了一些关于药用植物基因操纵的未来如何以实现更高的次生代谢产物产量为目标的评论。
This review describes the different plant transformation techniques, including guided infection withAgrobacterium tumefaciensandA. rhizogenes, particle bombardment and protoplast fusion, that have been attempted to create transgenicCatharanthus roseus(L.) G. Don cell cultures, hairy roots and whole plants. The review also focuses on the different approaches used to manipulate and improve secondary metabolite yields in various culture systems, with special attention to the most relevant results achieved. Finally, under future perspectives, the authors propose several approaches which would likely be implemented with this species, to try to boost the accumulation of the anti-tumour agents, vinblastine and vincristine. Some comments on how the future of the genetic manipulation of medicinal plants may proceed aiming at achieving higher secondary metabolite yields are also given.