DIRECT MEASUREMENT OF THE FORCE OF MICROTUBULE SLIDING IN FLAGELLA
DIRECT MEASUREMENT OF THE FORCE OF MICROTUBULE SLIDING IN FLAGELLA
复制标题
DOI：
10.1038/293566a0
复制
发表时间：
1981-01-01
期刊：
影响因子：
64.8
通讯作者：
TAKAHASHI, K
中科院分区：
文献类型：
--
作者：
KAMIMURA, S;TAKAHASHI, K
真核生物纤毛和鞭毛的运动是由atp驱动的双微管之间的主动滑动引起的1 - 3，这种滑动的力被认为是由a小管的动力蛋白臂与相邻双小管的b小管相互作用产生的。为了理解这种力产生反应的机械化学基础，并将其与纤毛或鞭毛的明显运动行为联系起来，量化动力臂施加的力是很重要的。已经有人尝试从整个细胞器产生的弯矩来估计这种力4,5，但估计必然是假设的，因为滑动与弯曲耦合的机制尚不清楚。如果用含有ATP的溶液灌注胰蛋白酶-2或弹性蛋白酶- 6处理的轴突，可以在体外诱导微管滑动而不弯曲。通过将玻璃微针连接到轴突上，我们现在已经直接确定了不同ATP浓度下的滑动力。
The movement of eukaryotic cilia and flagella is caused by ATP-driven active sliding between the doublet microtubules1–3, and the force for the sliding is believed to be generated by the dynein arms of the A-tubule interacting with the B-tubule of the adjoining doublet. To understand the mechanochemical basis of this force-generating reaction and to correlate it with the overt motile behaviour of cilia or flagella, it is important to quantify the force exerted by the dynein arms. Attempts have been made to estimate this force from the bending moment generated by the whole organelle4,5, but the estimation was of necessity hypothetical because the mechanism by which sliding is coupled with bending is poorly understood. Microtubule sliding without bending can be inducedin vitroif trypsin-2or elastase6-treated axonemes are perfused with a solution containing ATP. By attaching glass microneedles to such axonemes, we have now directly determined the sliding force at various ATP concentrations.