Classification of Pacemaker Dynamics in the Mouse Intestine by Field Potential Microimaging
Classification of Pacemaker Dynamics in the Mouse Intestine by Field Potential Microimaging
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通过场电位显微成像对小鼠肠道起搏器动力学进行分类
DOI：
10.1016/j.biosx.2022.100111
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
S.
中科院分区：
文献类型：
--
作者：
Iwata;N.;Takai;C.;Mochizuki;N.;Yamauchi;M.;Kaji;N.;Kasahara;Y.;Nakayama;S.
胃肠道（GI）的灵活和复杂的运动意味着肠神经回路以外的机制的参与，以协调微区域的兴奋。因此，我们在小鼠小肠中进行了起搏器动力学的显微成像，因为它包含典型的网络形成起搏器细胞。透析膜增强的低阻抗微电极阵列（MEA），使场电位在很宽的频率范围内，以稳定地测量在微区。起搏器动力学被分类为基本模式，尽管变化很大。在发育过程中，起搏器活动被分类为“扩展”或“迁移”模式，分别在MEA感知区域启动或传播。体积导体的细胞间电流使这两种活动的波形复杂化。“扩展”和“迁移”模式的存在是由于重复的起搏系统，如细胞内钙振荡激活和电压门控机制。此外，从起搏器事件期间的时空特征来看，“颠簸/异常”模式由异常的、不连贯的传播定义，并与局部兴奋性受损相关，而“碰撞/会聚”模式涉及MEA区域中多种活动的相互作用。四种微配位模式之间的相互转换发生在同一微区。5-羟色胺（5-HT）促进“迁移”活动，这意味着空间电导率的改善或恢复。这些结果与5-HT改变胃肠道运动向推进方向的作用一致。总之，我们的微成像分类MEA方法能够定量评估胃肠道运动的时空电协调，这表明其适用于小型模型动物。
The flexible and sophisticated movement of the gastrointestinal (GI) tract implies the involvement of mechanisms other than enteric neural circuits, to coordinate excitation in microregions. We thus performed microimaging of pacemaker dynamics in the small intestine of mice since it contains typical network-forming pacemaker cells. A dialysis membrane-reinforced low-impedance microelectrode array (MEA) enabled field potentials over a wide frequency range to be stably measured in microregions. The pacemaker dynamics were classified into basic patterns despite large variations. In the developmental process, pacemaker activity was categorized as either an ‘expanding’ or a ‘migrating’ pattern that was initiated in or propagated to the MEA sensing area, respectively. The intercellular current of the volume conductor complicated the waveform of both activities. The existence of ‘expanding’ and ‘migrating’ patterns was attributable to duplicated pacemaker systems such as intracellular Ca2+oscillation-activated and voltage-gated mechanisms. Additionally, from the spatio-temporal feature during the period of pacemaker events, the ‘bumpy/aberrant’ pattern was defined by aberrant, incoherent propagation, and associated with local impairment of excitability, while the ‘colliding/converging’ pattern involved the interaction of multiple activities in the MEA area. Interconversion between the four micro-coordination patterns occurred in the same microregion. 5-Hydroxytryptamine (5-HT) promoted ‘migrating’ activity, implying an improvement or restoration of spatial conductivity. These results agree well with the action of 5-HT to change GI movement toward propulsion. In conclusion, our MEA method of microimaging classification enables the quantitative assessment of spatio-temporal electric coordination underlying GI motility, suggesting its application to small model animals.
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DOI：
--
发表时间：
2003
期刊：
Journal of Physiology
影响因子：
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DOI：
10.1038/nrgastro.2017.15
发表时间：
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期刊：
Nature Reviews Gastroenterology &Hepatology
影响因子：
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