Microsatellite markers as a tool for active germplasm management and bridging the gap between national and local collections of apple
Microsatellite markers as a tool for active germplasm management and bridging the gap between national and local collections of apple
复制标题
微卫星标记作为主动种质管理和缩小国家和地方苹果收藏之间差距的工具
DOI：
10.1007/s10722-022-01342-5
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发表时间：
2022
影响因子：
2
通讯作者：
M. Ordidge
中科院分区：
文献类型：
--
作者：
E. Venison;S. Litthauer;Peter Laws;C. Denancé;F. Fernández;C. Durel;M. Ordidge
简单序列重复（SSR）微卫星标记广泛应用于苹果种质资源的重复鉴定和遗传多样性分析。在这里，我们展示了在一个重要的国际种质收集——英国国家水果收集（NFC）的管理再繁殖中使用一套标准SSR位点的发现。8个SSR位点的一个子集被认为足以在1995年的样本中区分所有克隆亲属，只有一对完整的兄弟姐妹例外。总体而言，99%的材料在替换了最初鉴定为错误繁殖的树木后能够被确认存在和正确。在收藏本身的管理的同时，通过英国当地苹果爱好者社区领导的一项倡议，将当地收藏的苹果的3373个SSR档案与NFC馆藏进行了比较。总体而言，两组材料的多样性都保持在较高水平，NFC馆藏和本地馆藏的平均基因多样性值分别为0.800和0.812。尽管发现了大量具有本地价值和遗传可区分的个体，其中一些可能代表“丢失”的品种，但当地收集的材料在遗传多样性的总体覆盖率方面与NFC收集的材料没有差异（FST = 0.0035）。
Simple sequence repeat (SSR) microsatellite markers have been extensively used to identify duplication and analyse genetic diversity in germplasm collections of apple. Here, we present findings from the use of a standard set of SSR loci in the managed repropagation of a significant international germplasm collection: the UK National Fruit Collection (NFC). A subset of eight SSR loci was deemed sufficient to distinguish all apart from the clonal relatives across a sample of 1995 accessions, with a single exception being one pair of full siblings. In total, 99% of accessions were able to be confirmed present and correct after the replacement of trees initially identified to be incorrectly propagated. In parallel to the curation of the collection itself, through an initiative led by the UK local apple enthusiast community, 3373 SSR profiles for apples held in local collections were compared to the NFC holdings. Overall, in both sets of material, diversity remained high with average gene diversity values of 0.800 and 0.812 in the NFC holdings and local collections, respectively. Accessions in local collections were not found to differ in their overall coverage of genetic diversity to that of the NFC collection (FST = 0.0035) although significant numbers of locally valued, and genetically distinguishable individuals were identified, some of which may represent ‘lost’ cultivars.
影响因子：
3.3
作者：
L. Excoffier;P. Smouse;J. Quattro
通讯作者：
L. Excoffier;P. Smouse;J. Quattro