Tubulin polymerization modulates interleukin-2 receptor signal transduction in human T cells.
Tubulin polymerization modulates interleukin-2 receptor signal transduction in human T cells.
复制标题
微管蛋白聚合调节人 T 细胞中的 IL-2 受体信号转导。
DOI：
10.1080/10799890600567372
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Roszman,ThomasL
中科院分区：
文献类型：
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作者：
Goebel,Jens;Forrest,Kathy;Wills-Karp,Marsha;Roszman,ThomasL
关于肌动蛋白或微管蛋白细胞骨架调节细胞因子受体信号传导的数据很少。因此，我们在细胞松弛素 D (CyD)、jasplaklinolide (Jas)、紫杉醇 (Tax) 或秋水仙碱 (Col) 诱导的细胞骨架系统改变的背景下，研究了经植物血凝素 (PHA) 预处理的人 T 细胞中白细胞介素 2 受体 (IL-2R) 信号传导。我们发现细胞骨架微管蛋白聚合的变化改变了几种 IL-2 触发信号的强度。此外，Tax 诱导的微管蛋白高聚合增强了 IL-2R β 链的表面表达，并增强了 IL-2R γ 链与细胞骨架微管蛋白的关联。 IL-2R β 链又与微管蛋白以及更弱的肌动蛋白组成性相关。为了排除这些关联是由 PHA 引起的伪影的可能性，我们在用标称抗原刺激的 TCR 转基因 DO11.10 小鼠的 T 细胞中证实了它们。我们得出的结论是，细胞骨架成分（尤其是微管蛋白）聚合的改变伴随着受体水平上 IL-2 信号传导的调节。
Few data exist on the modulation of cytokine receptor signaling by the actin or tubulin cytoskeleton. Therefore, we studied interleukin-2 receptor (IL-2R) signaling in phytohemagglutinine (PHA)-pretreated human T cells in the context of alterations in the cytoskeletal system induced by cytochalasin D (CyD), jasplaklinolide (Jas), taxol (Tax), or colchicine (Col). We found that changes in cytoskeletal tubulin polymerization altered the strength of several IL-2-triggered signals. Moreover, Tax-induced tubulin hyperpolymerization augmented the surface expression of the IL-2R β -chain and enhanced the association of the IL-2R γ -chain with cytoskeletal tubulin. The IL-2R β -chain, in turn, was constitutively associated with tubulin and, more weakly, actin. To exclude the possibility that these associations are artifacts caused by PHA, we confirmed them in T cells from TCR-transgenic DO11.10 mice stimulated with their nominal antigen. We conclude that altered polymerization of cytoskeletal components, especially tubulin, is accompanied by modulation of IL-2 signaling at the receptor level.