Placental apoptosis in preeclampsia
Placental apoptosis in preeclampsia
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DOI：
10.1016/s0029-7844(00)00895-4
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发表时间：
2000-08-01
影响因子：
7.2
通讯作者：
Lessey, BA
中科院分区：
文献类型：
--
作者：
Allaire, AD;Ballenger, KA;Lessey, BA
目的：为了确定是否先兆子痫是与胎盘细胞凋亡和差异表达的介质apoptosis.Methods：胎盘样本从31先兆子痫妇女和31正常血压对照组进行了分析，使用末端脱氧核苷酸转移酶介导的脱氧尿苷三磷酸缺口末端标记染色。Pas，Fas配体，Bcl-2和Bar的表达进行了评估，使用免疫组化。结果：研究组的凋亡细胞核的中位数百分比显著高于对照组（0.49比0.19; P = .001），作为滋养层细胞核的凋亡细胞核的中位数百分比（0.33比0.09; P < .01）。与对照组相比，研究受试者绒毛滋养层中Pas配体表达显著减少，Fas表达显著增加。结论：胎盘细胞凋亡及滋养细胞Fas、Pas配体表达的改变可能影响子痫前期的发病机制及后遗症。((C)2000年由美国妇产科学院出版）。
Objective: To determine whether preeclampsia is associated with an increase in placental apoptosis and differential expression of mediators of apoptosis.Methods: Placental samples from 31 preeclamptic women and 31 normotensive controls were analyzed using terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling staining. Expression of Pas, Fas ligand, Bcl-2, and Bar was assessed using immunohistochemistry.Results: The median percent apoptotic nuclei was significantly higher for the study group than for the controls (0.49 versus 0.19; P = .001), as was the median percent apoptotic nuclei in the trophoblast nuclei (0.33 versus 0.09; P < .01). Pas ligand expression was significantly less and Fas expression significantly greater in the villus trophoblast among the study subjects compared with controls. There was no difference in the expression of Pax or Bcl-2 between groups.Conclusion: Placental apoptosis and altered expression of Fas and Pas ligand in trophoblast might influence pathogenesis or sequelae of preeclampsia. ((C) 2000 by The American College of Obstetricians and Gynecologisis.).