Single-particle tracking of endocytosis and exocytosis of single-walled carbon nanotubes in NIH-3T3 cells
Single-particle tracking of endocytosis and exocytosis of single-walled carbon nanotubes in NIH-3T3 cells
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DOI：
10.1021/nl072969s
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发表时间：
2008-06-01
期刊：
影响因子：
10.8
通讯作者：
Strano, Michael S.
中科院分区：
文献类型：
--
作者：
Jin, Hong;Heller, Daniel A.;Strano, Michael S.
超过10000个单独的轨迹nonphotobleaching单壁碳纳米管（单壁碳纳米管）被跟踪，因为它们被纳入和驱逐NIH-3 T3细胞在真实的时间灌注显微镜阶段。均方位移的分析允许从单持续时间实验所涉及的机械步骤的完整建设。我们观察到的第一个确凿的证据，单壁碳纳米管的胞吐作用，并表明，该速率密切匹配的内吞率可以忽略不计的时间偏移。我们确定和研究的内吞和胞吐途径，导致先前观察到的聚集和积累的单壁碳纳米管的细胞内。
Over 10000 individual trajectories of nonphotobleaching single-walled carbon nanotubes (SWNT) were tracked as they are incorporated into and expelled from NlH-3T3 cells in real time on a perfusion microscope stage. An analysis of mean square displacement allows the complete construction of the mechanistic steps involved from single duration experiments. We observe the first conclusive evidence of SWNT exocytosis and show that the rate closely matches the endocytosis rate with negligible temporal offset. We identify and study the endocytosis and exocytosis pathway that leads to the previously observed aggregation and accumulation of SWNT within the cells.