Paragonimus skrjabini Chen, 1959 (Digenea: Paragonimidae) and related species in eastern Asia:: a combined molecular and morphological approach to identification and taxonomy
Paragonimus skrjabini Chen, 1959 (Digenea: Paragonimidae) and related species in eastern Asia:: a combined molecular and morphological approach to identification and taxonomy
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DOI：
10.1007/s11230-004-1378-5
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发表时间：
2005-01-01
影响因子：
1.3
通讯作者：
Ximei, Z
中科院分区：
文献类型：
--
作者：
Blair, D;Zhengshan, C;Ximei, Z
本文报道了对哺乳动物肺吸虫种复合体的分子和形态计量学研究，其中斯氏并殖吸虫（Paragonimus skrjabini Chen，1959）和宫崎并殖吸虫（P. miyazakii卡莫，Nishida，Hatsushika & Tomimura，1961）是最著名的两个例子。使用来自核ITS 2区和线粒体考克斯]基因的DNA序列，该种复合体（这里称为斯氏红蜘蛛复合体）被证明是单系的。后一个标记允许人口的歧视，一些以前命名为不同的物种，从不同的地理位置在中国和日本。一些变量的形态计量学分析符合非常密切的分子结果。主要研究结果如下：（1）福建省的斯氏原蜱与日本的宫崎原蜱在遗传学上非常接近。这两个分类群应与宫崎拟步行虫同属一个亚种。(2)广东、广西、四川、湖北等地的种群应统称为斯氏狸殖吸虫。根据遗传学和形态测量学数据，来自四川和湖北的长江流域的斯氏狸殖吸虫标本彼此非常相似，但与广东种群的差异不大，因此应将其归为不同的亚种。云南的P skrjabini标本在此阶段尚未被指定为一个亚种。(3)部分根据早期的研究，我们认为以下是P skrjabini的异名：R miyazakii（降低到亚种地位）; P szechuanensis Chung & Tsao，1962（可能属于R s. skriabini）;和惠顿拟青霉P. hueitungensis Chung，Hsu，Ho，Kao，Shao，Chin，Pi，Liu，Ouyang，Shen，Yi & Yao，1975（可能属于P.s. skriabini）; P. veocularis（Chen & Li，1979）（来自福建的标本被认为属于本种应该被称为R skriabini miyazakii：来自北方四川的模式产地的标本的亚种状态不清楚）。一些问题仍未解决。云南南部两个形态独特的囊蚴被命名为P hokuoensis Ho & Chung，1964。来自同一地点的非常相似的囊蚴的DNA序列将这个名义物种置于斯氏狸殖吸虫复合体内或姐妹。到目前为止，没有什么是已知的关于成年形态学或生物学这分类群，我们保留它在这里作为一个独特的种。异睾吸虫（Zhou、Pang和Hsiang，1982）可能是斯氏锥虫的同义词：囊蚴的形式提供了反对这一观点的证据，需要进一步的工作。在中国，长喙拟青霉（P. macrabensis Chen，1962）可能与斯氏拟青霉（P. skrjabini）混淆。在中国，前者可能仅分布于海南岛，而福建省的P. fukienensis Tang & Tang，1962可能是其异名。
A molecular and morphometric investigation is reported on the species complex of mammalian lungflukes of which Paragonimus skrjabini Chen, 1959 and P. miyazakii Kamo, Nishida, Hatsushika & Tomimura, 1961 are the best-known examples. This species complex (here called the R skrjabini complex) is shown to be monophyletic using DNA sequences from the nuclear ITS2 region and the mitochondrial cox] gene. The latter marker permits the discrimination of populations, some previously named as distinct species, from various geographical locations in China and Japan. Morphometric analysis of a number of variables accords remarkably closely with the molecular results. Main findings are that: (1) nominal P. skrjabini from Fujian Province in eastern China is phylogenetically very close to P. miyazakii from Japan. It is proposed that both taxa should be refer-red to the same subspecies as P. skrjabini miyazakii. (2) Populations from Guangdong Province, Guangxi Zhuang Autonomous Region, Sichuan and Hubei should be referred to P. skrjabini skrjabini. Specimens of P. skriabini from the Yangtze basin in Sichuan and Hubei are remarkably similar to one another according to genetic and morphometric data, but are not so different from Guangdong populations that they should be referred to a different subspecies at this stage. Specimens of P skrjabini from Yunnan have not been assigned to a subspecies at this stage. (3) Partly following earlier studies, we regard the following as synonyms of P skrjabini: R miyazakii (reduced to subspecific status); P. szechuanensis Chung & Tsao, 1962 (probably belongs to R s. skriabini); and P. hueitungensis Chung, Hsu, Ho, Kao, Shao, Chin, Pi, Liu, Ouyang, Shen, Yi & Yao, 1975 (probably belongs to P. s. skriabini); P. veocularis (Chen & Li, 1979) (specimens from Fujian regarded as belonging to this species should be referred to R skriabini miyazakii: the subspecific status of specimens from the type-locality in northern Sichuan is unclear). A number of questions remain unresolved. The name P hokuoensis Ho & Chung, 1964 was proposed for two individual metacercariae of distinctive appearance from southern Yunnan. DNA sequences from very similar metacercariae from the same locality place this nominal species within, or sister to, the P. skrjabini complex. As yet, nothing is known regarding adult morphology or biology of this taxon and we retain it here as a distinct species. P. heterorchis (Zhou, Pang & Hsiang, 1982) might be a synonym of R skriabini: the form of the metacercaria provides evidence against this view and further work is required. P. macrorchis Chen, 1962 has probably been confused with P. skrjabini in China. Within China, the former probably occurs only on Hainan Island, although P. fukienensis Tang & Tang, 1962 from Fujian Province could be a synonym.