Functional characterization of osteosarcoma cell lines provides representative models to study the human disease
Functional characterization of osteosarcoma cell lines provides representative models to study the human disease
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DOI：
10.1038/labinvest.2011.72
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发表时间：
2011-08-01
影响因子：
5
通讯作者：
Cleton-Jansen, Anne-Marie
中科院分区：
文献类型：
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作者：
Mohseny, Alexander B.;Machado, Isidro;Cleton-Jansen, Anne-Marie
癌细胞系代表了研究恶性肿瘤、一般细胞生物学、药物发现等的体外模型。考虑到获得额外的细胞离体变化以及缺乏组织结构和基质，它们是否可以被认为是亲代肿瘤的确切代表性模型仍然不确定。以前，在EuroBoNeT联盟内，我们在基因组和蛋白质组水平上表征了骨肉瘤细胞系的集合。在这里，我们解决的表型和功能特性的独特的一套骨肉瘤细胞系（n = 19）在体外和体内。对于分化能力的功能分析，细胞被刺激朝向成骨细胞、脂肪细胞和软骨细胞。此外，将所有细胞系皮下和肌内注射到裸鼠中，以测定其体内肿瘤形成能力以及用于肿瘤的表型分析。所有形成的肿瘤进一步进行了组织学和免疫化学表征。在19个细胞系中，17个（89%）显示成脂分化，13/19个（68%）可以向成骨细胞分化，并且在6/19个（32%）细胞系中软骨分化是明显的。约一半的细胞系（8/19，42%）在皮下和肌内注射后体内产生肿瘤。几个细胞系表现出对邻近组织的侵袭，并且一个肿瘤发展了几个肺转移。细胞系的使用，特别是在癌症研究中，是至关重要的。在这里，我们确定了全面表征的骨肉瘤细胞系，这有力地代表了临床骨肉瘤，为研究人员提供了有用的体外和体内模型，以研究这种高度恶性肿瘤的遗传学和功能特征。实验室调查（2011）91，1195-1205; doi：10.1038/labinvest.2011.72; 2011年4月25日在线发表
Cancer cell lines represent in vitro models for studying malignancies, general cell biology, drug discovery and more. Whether they can be considered as exact representative models of the parental tumors remains uncertain given the acquisition of additional ex vivo changes of the cells and the lack of tissue architecture and stroma. Previously, within the EuroBoNeT consortium, we characterized a collection of bone sarcoma cell lines on genomic and proteomic level. Here, we address the phenotypical and functional characterization of the unique set of osteosarcoma cell lines (n = 19) in vitro and in vivo. For functional analysis of differentiation capacity, cells were stimulated towards osteoblasts, adipocytes and chondrocytes. Furthermore, all cell lines were injected subcutaneously and intramuscularly into nude mice to assay their in vivo tumor formation capacity as well as for phenotypical analysis of the tumors. All formed tumors were further characterized histologically and immunohistochemically. Out of 19 cell lines, 17 (89%) showed adipogenic differentiation, 13/19 (68%) could differentiate towards osteoblasts and in 6/19 (32%) cell lines chondrogenic differentiation was evident. About half of the cell lines (8/19, 42%) produced tumors in vivo after subcutaneous and intramuscular injections. Several cell lines showed invasion into adjacent tissues and one tumor developed several lung metastases. The use of cell lines, especially in cancer research, is of paramount importance. Here, we identify comprehensively characterized osteosarcoma cell lines, which robustly represent clinical osteosarcoma providing researchers useful in vitro and in vivo models to study the genetics and functional characteristics of this highly malignant neoplasm. Laboratory Investigation (2011) 91, 1195-1205; doi:10.1038/labinvest.2011.72; published online 25 April 2011