A 7.5 Mb sequence-ready PAC contig and gene expression map of human chromosome 11p13-p14.1
A 7.5 Mb sequence-ready PAC contig and gene expression map of human chromosome 11p13-p14.1
复制标题
DOI：
10.1101/gr.9.11.1074
复制
发表时间：
1999-11-01
期刊：
影响因子：
7
通讯作者：
Gessler, M
中科院分区：
文献类型：
--
作者：
Gawin, B;Niederführ, A;Gessler, M
人类11号染色体短臂的p13区域在寻找与肾母细胞瘤、无虹膜、泌尿生殖系统异常、精神发育迟滞（WAGR）综合征和相关疾病的病因有关的基因的过程中得到了深入研究。这个区域的基因图谱还远未完成，然而，加强了对额外基因鉴定工作的需求。我们描述了扩展现有的重叠群地图与PI衍生的人工染色体（PAC）覆盖7.5 Mb的11 p13 -14.1。通过末端探针步移和指纹分析建立了扩展的序列就绪重叠群，该重叠群由201个PAC克隆组成。利用重叠PAC定义的法案，我们生成了一个详细的基因图谱，其中包含20个基因以及22个匿名EST，这些EST已经通过搜索RH数据库确定。相对湿度图和我们建立的基因图显示了全球相关性，但目前的相对湿度面板的分辨率的限制是显而易见的，在这个规模。通过北方印迹分析对新基因进行了初步表达研究。为了扩展这些表达谱，通过数据库搜索鉴定相应的小鼠cDNA克隆，并用于小鼠胚胎切片的北方印迹分析和RNA原位杂交。克隆的基因组测序沿着一个最小的平铺路径通过重叠群目前正在进行中，并将促进这些表达的研究，在电脑基因鉴定方法。
The region p13 of the short arm of human chromosome 11 has been studied intensely during the search for genes involved in the etiology of the Wilms' tumor, aniridia, genitourinary abnormalities, mental retardation (WAGR) syndrome, and related conditions. The gene map for this region is far from being complete, however, strengthening the need For additional gene identification efforts. We describe the extension of an existing contig map with Pi-derived artificial chromosomes (PACs) to cover 7.5 Mb of 11p13-14.1. The extended sequence-ready contig was established by end probe walking and fingerprinting and consists of 201 PAC clones. Utilizing bills defined by overlapping PACs, we generated a detailed gene map containing 20 genes as well as 22 anonymous ESTs which have been identified by searching the RH databases. RH maps and our established gene map show global correlation, but the limits of resolution of the current RH panels are evident at this scale. Initial expression studies on the novel genes have been performed by Northern blot analyses. To extend these expression profiles, corresponding mouse cDNA clones were identified by database search and employed For Northern blot analyses and RNA in situ hybridizations to mouse embryo sections. Genomic sequencing of clones along a minimal tiling path through the contig is currently under way and will facilitate these expression studies by in silico gene identification approaches.