Role of DNA methylation in hybrid vigor in Arabidopsis thaliana
Role of DNA methylation in hybrid vigor in Arabidopsis thaliana
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DOI：
10.1073/pnas.1613372113
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发表时间：
2016-10-25
影响因子：
11.1
通讯作者：
Fujimoto, Ryo
中科院分区：
文献类型：
--
作者：
Kawanabe, Takahiro;Ishikura, Sonoko;Fujimoto, Ryo
杂种优势或杂种优势是指杂种F1代植株表现出的上级性状超过其亲本。杂种优势在主要作物的生产系统中特别重要。近年来的研究表明，DNA甲基化等表观遗传调控参与了杂种优势的形成，但杂种优势形成的分子机制尚不清楚。为了解决拟南芥杂种优势的表观遗传贡献，我们使用了在DNA甲基化中起作用的突变基因。没有RNA聚合酶IV（Pol IV）或甲基转移酶I（MET 1）功能的C24和哥伦比亚-0（Col）之间的杂交没有降低生物量杂种优势的水平（如通过莲座直径评估的）。具有dna甲基化减少1（ddm 1）突变的杂种表现出较低的杂种优势水平。ddm 1突变体杂种的营养杂种优势降低，但没有消除;如果在产生杂种优势水平的所有关键基因座的甲基化发生变化，则可能导致完全降低，而如果只有一部分基因座发生甲基化变化，则可能仅部分降低杂种优势。
Hybrid vigor or heterosis refers to the superior performance of F-1 hybrid plants over their parents. Heterosis is particularly important in the production systems of major crops. Recent studies have suggested that epigenetic regulation such as DNA methylation is involved in heterosis, but the molecular mechanism of heterosis is still unclear. To address the epigenetic contribution to heterosis in Arabidopsis thaliana, we used mutant genes that have roles in DNA methylation. Hybrids between C24 and Columbia-0 (Col) without RNA polymerase IV (Pol IV) or methyltransferase I (MET1) function did not reduce the level of biomass heterosis (as evaluated by rosette diameter). Hybrids with a mutation in decrease in dna methylation 1 (ddm1) showed a decreased heterosis level. Vegetative heterosis in the ddm1 mutant hybrid was reduced but not eliminated; a complete reduction could result if there was a change in methylation at all loci critical for generating the level of heterosis, whereas if only a proportion of the loci have methylation changes there may only be a partial reduction in heterosis.