Impairment of early neuronal maturation in anti-NMDA-receptor encephalitis.
Impairment of early neuronal maturation in anti-NMDA-receptor encephalitis.
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抗 NMDA 受体脑炎中早期神经元成熟受损。
DOI：
10.1007/s00213-021-06036-x
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Yamada N.
中科院分区：
文献类型：
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作者：
Okamoto S;Takaki M;Hinotsu K;Kawai H;Sakamoto S;Okahisa Y;Takao S;Tsutsui K;Kanbayashi T;Tanaka K;Yamada N.
妊娠期抗NMDAR脑炎的适当免疫治疗对孕妇及其婴儿产生了相对良好的临床结局，但尚无关于其婴儿未来生长的报告。抗NMDAR抗体对早期神经元发育的损害仍然是未知的。ObjectivesSerum或脑脊液从一个病人与抗NMDAR脑炎（索引患者）和一个病人与精神分裂症（对照患者）被管理到原代培养的分离大鼠皮层神经元，树突的生长，中心体消除，和树突的分支进行了调查。对于救援实验，血清的指数患者被替换为正常的培养基后3天的管理指数patient.Resultsserum和脑脊液的指数患者的统计学显着受损的树突状生长培养的大鼠皮层原代神经元。索引患者的血清也在统计学上显著延迟中心体消除。受损的树突状细胞生长和延迟的中心体消除并没有通过改变为正常的培养基完全获救。指标患者的血清也在统计学上显着减少了树突的分支。结论这是首次证明抗NMDAR抗体对体外早期树突发育的损害。作为一种保护胚胎神经元的策略，我们的研究结果可能支持妊娠期抗NMDAR脑炎的早期免疫治疗的有效性。
RationaleAdequate immunotherapies for anti-NMDAR encephalitis during pregnancy produce a relatively good clinical outcome for pregnant mothers and their infants, but there are no reports about the future growth of their babies. The damage of anti-NMDAR antibodies to early neuronal development is still unknown.ObjectivesSerum or cerebrospinal fluid from one patient with anti-NMDAR encephalitis (the index patient) and one patient with schizophrenia (the control patient) was administered to primary cultures of dissociated rat cortical neurons, and dendritic outgrowth, centrosome elimination, and branching of dendrites were investigated. For rescue experiments, serum of the index patient was replaced with normal culture media after 3 days’ administration of the index patient.ResultsSerum and cerebrospinal fluid of the index patient statistically significantly impaired dendritic outgrowth of cultured rat cortical primary neurons. Serum of the index patient also statistically significantly delayed centrosome elimination. Impaired dendritic outgrowth and delayed centrosome elimination were not perfectly rescued by changing to normal culture media. Serum of the index patient also statistically significantly reduced the branching of dendrites.ConclusionsThis is the first demonstration of the damage by anti-NMDAR antibodies on early dendritic development in vitro. As a strategy to protect embryonic neurons, our findings may support the efficacy of early immunotherapy for anti-NMDAR encephalitis in pregnancy.