Using Flow Cytometry to Analyze Cryptococcus Infection of Macrophages.
Using Flow Cytometry to Analyze Cryptococcus Infection of Macrophages.
复制标题
使用流式细胞术分析巨噬细胞的隐球菌感染。
DOI：
10.1007/978-1-4939-6581-6_24
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Evans RJ
中科院分区：
文献类型：
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作者：
Evans RJ
流式细胞术是一种强大的分析技术，越来越多地用于研究宿主细胞与细胞内病原体之间的相互作用。与荧光显微镜等替代技术相比，流式细胞术能够测量样品中更多数量的感染细胞。这意味着可以对感染期间的罕见事件进行可靠的量化。我们的实验室和其他人已经开发了流式细胞术方法来研究宿主细胞和细胞内病原体之间的相互作用，如新生隐球菌，以量化吞噬作用，细胞内复制和非溶解性驱逐或“呕吐”从吞噬体。在这里，我们描述了这些方法，以及如何将它们应用于C。neoformanas以及其他类似的细胞内病原体。
Flow cytometry is a powerful analytical technique, which is increasingly being used to study the interaction between host cells and intracellular pathogens. Flow cytometry is capable of measuring a greater number of infected cells within a sample compared to alternative techniques such as fluorescence microscopy. This means that robust quantification of rare events during infection is possible. Our lab and others have developed flow cytometry methods to study interactions between host cells and intracellular pathogens, such asCryptococcus neoformans, to quantify phagocytosis, intracellular replication, and non-lytic expulsion or “vomocytosis” from the phagosome. Herein we describe these methods and how they can be applied to the study ofC. neoformansas well as other similar intracellular pathogens.
DOI：
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发表时间：
2010
期刊：
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影响因子：
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作者：
Kerstin Voelz;S. Johnston;Julian C Rutherford;R. May
通讯作者：
Kerstin Voelz;S. Johnston;Julian C Rutherford;R. May