Natalizumab has no direct biological effect on JC virus infectivity in permissive human neural cell lines
Natalizumab has no direct biological effect on JC virus infectivity in permissive human neural cell lines
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那他珠单抗对允许的人类神经细胞系中的 JC 病毒感染性没有直接的生物学作用
作者：
Suzuki T;Yamanouchi S;Sunden Y;Orba Y;Kimura T;Sawa H
人类多瘤病毒 JC 病毒 (JCV) 感染 70-80% 的人类，并在肾脏中建立潜伏感染。在免疫抑制患者中，JCV 重新激活并导致致命的进行性神经系统疾病，称为进行性多灶性白质脑病 (PML)。在过去的三十年中，PML 已成为获得性免疫缺陷综合征（AIDS）患者的重要神经并发症。最近，有报道称，接受针对整合素受体极晚期抗原 (VLA)-4 的治疗的患者发生 PML 的风险增加。然而，那他珠单抗与 PML 意外发作之间的关系尚未阐明。在这里，我们研究了那他珠单抗对病毒接种后允许的人类神经细胞系 IMR-32 中 JCV 生长的影响。那他珠单抗对使用特异性抗体进行免疫印迹分析测定的病毒蛋白的表达水平或对感染细胞的细胞裂解物的血凝活性没有影响。这些结果表明那他珠单抗对体外 IMR-32 细胞中的 JCV 感染性没有直接影响。 J.医学。病毒。 82：1229–1235，2010。© 2010 Wiley‐Liss, Inc.
The human polyomavirusJC virus (JCV) infects 70–80% of humans and establishes latent infection in the kidney. In immunosuppressed patients, JCV reactivates and causes a fatal and progressive neurological disease known as progressive multifocal leukoencephalopathy (PML). Over the past three decades, PML has become an important neurological complication in acquired immunodeficiency syndrome (AIDS) patients. Recently, it was reported that patients treated with therapeutics that target the integrin receptor very late antigen (VLA)‐4 are at increased risk of developing PML. However, the relationship between Natalizumab and this unexpected onset of PML has yet to be elucidated. Here, we investi‐gated the effect of Natalizumab on the growth of JCV in the permissive human neural cell line IMR‐32 following viral inoculation. Natalizumab had no effect either on the expression levels of viral proteins as determined by immunoblot analysis using specific antibodies or on the hemagglutination activity of cellular lysates from infected cells. These results suggest that there is no direct effect of Natalizumab on JCV infectivity in IMR‐32 cells in vitro. J. Med. Virol. 82: 1229–1235, 2010. © 2010 Wiley‐Liss, Inc.