A simple procedure for standard additions in flow injection analysis : Spectrophotometric Determination of Nitrate in Plant Extracts
A simple procedure for standard additions in flow injection analysis : Spectrophotometric Determination of Nitrate in Plant Extracts
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流动注射分析中标准添加的简单程序：植物提取物中硝酸盐的分光光度测定
DOI：
10.1016/s0003-2670(00)85586-5
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发表时间：
1983
影响因子：
6.2
通讯作者：
A. O. Jacintho
中科院分区：
文献类型：
--
作者：
M. Giné;B. Reis;E.A.G. Zaatto;F. Krug;A. O. Jacintho
如果使用区域采样从已知标准的分散区中选择具有不同浓度水平的区段，并且这些区段与样品区合并，则在流动注射系统中容易实现恒定样品体积的标准加入方法。该系统需要单一的标准溶液，适用于常规的大规模分析，不需要复杂的设备。根据所需的采样速率、方法的速度和样品中的平均分析物含量来选择添加的顺序和水平。讨论了系统的设计细节、稳定性和潜力。作为应用，该体系用于植物提取物中硝酸盐的光度测定，需要用标准加入法来克服基质的影响。基于每个样本12次添加的结果(大约每小时100次测量)，结果与通过相应的手动程序获得的结果一致。
The standard additions method with constant sample volumes is readily achieved in a flow injection system if zone sampling is used to select segments with different concentration levels from the dispersed zone of a known standard, and these segments are merged with the sample zone. This system requires a single standard solution, is suitable for routine large-scale analyses, and does not need complex equipmnt. The number of sequence and level of additions are chosen in accordance with the required sampling rate, the speed of the method and the mean analyte content in the samples. Details of system design, stability and potential are discussd. As an applicaion, this system was used in the spectrophotometric determination of nitrate in plant extracts where the standard additions method is required to overcome the matrix effect. The results, based on twelve additions per sample (about 100 measurements per hour) agree with those obtained by the corresponding manual procedure.