Effect of Bacoside A on membrane-bound ATPases in the brain of rats exposed to cigarette smoke
Effect of Bacoside A on membrane-bound ATPases in the brain of rats exposed to cigarette smoke
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DOI：
10.1002/jbt.20050
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发表时间：
2005-01-01
影响因子：
3.6
通讯作者：
Devi, CSS
中科院分区：
文献类型：
--
作者：
Anbarasi, K;Vani, G;Devi, CSS
膜结合酶通过维持膜电位和脉冲传播在神经元功能中起着至关重要的作用。我们评估了慢性吸烟对大鼠脑中膜结合ATP酶的有害影响和Bacoside A的保护作用。将成年雄性白化病大鼠暴露于香烟烟雾中12周，同时以10 mg/kg B.w/天的剂量口服给予Bacoside A（从假马齿苋中分离的活性成分）。测定脑组织中脂质过氧化物水平，Na+/K+-ATP酶、Ca ~（2+）-ATP酶和Mg ~（2+）-ATP酶活性，以及Na+、K+、Ca ~（2+）和Mg ~（2+）离子含量。神经元膜损伤是明显的脂质过氧化物水平的升高和膜结合酶的活性降低。还观察到Na+和Ca 2+积累以及K+和Mg 2+耗尽的电解质平衡紊乱。Bacoside A可抑制脂质过氧化，提高ATP酶活性，维持离子平衡。我们的研究结果表明，Bacoside A保护大脑免受吸烟引起的膜损伤。(C)2005 Wiley Periodicals，Inc.
Membrane-bound enzymes play a vital role in neuronal function through maintenance of membrane potential and impulse propagation. We have evaluated the harmful effects of chronic cigarette smoking on membrane-bound ATPases and the protective effect of Bacoside A in rat brain. Adult male albino rats were exposed to cigarette smoke for a period of 12 weeks and simultaneously administered with Bacoside A (the active principle isolated from Bacopa monniera) at a dosage of 10 mg/kg b.w/day, p.o. The levels of lipid peroxides as marker for evaluating the extent of membrane damage, the activities of Na+/K+-ATPase, Ca2+-ATPase and Mg2+-ATPase, and associated cations sodium (Na+), potassium (K+), calcium (Ca2+), and magnesium (Mg2+) were investigated in the brain. Neuronal membrane damage was evident from the elevated levels of lipid peroxides and decreased activities of membrane-bound enzymes. Disturbances in the electrolyte balance with accumulation of Na+ and Ca2+ and depletion of K+ and Mg2+ were also observed. Administration of Bacoside A inhibited lipid peroxidation, improved the activities of ATPases, and maintained the ionic equilibrium. The results of our study indicate that Bacoside A protects the brain from cigarette smoking induced membrane damage. (C) 2005 Wiley Periodicals, Inc.