Study on the effect of the treatment of Periplaneta americana L. extract Ento-B by Dinitrochlorobenzene combined with acetic acid induced UC in rats.
Study on the effect of the treatment of Periplaneta americana L. extract Ento-B by Dinitrochlorobenzene combined with acetic acid induced UC in rats.
复制标题
美洲大蠊提取物Ento-B二硝基氯苯联合乙酸治疗大鼠UC的作用研究
DOI：
10.1590/acb360102
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发表时间：
2021
影响因子：
1.1
通讯作者：
Liu H
中科院分区：
文献类型：
--
作者：
Liu SS;Xu YS;Hilola A;Zhou N;He M;Zhang CG;Liu H
目的研究美洲大蠊（Periplaneta americana L.）目的：观察中药提取物Ento-B对2，4-二硝基氯苯和醋酸所致大鼠慢性溃疡性结肠炎的治疗作用，并探讨其作用机制。采用2，4-二硝基氯苯联合醋酸诱导大鼠慢性溃疡性结肠炎（chronic UC）模型。柳氮磺胺吡啶（400 mg/kg）和Ento-B以疾病活动指数（DAI）评分、结肠黏膜损伤指数（CMDI）评分、组织病理学评分及血清白细胞介素-4（IL-4）、白细胞介素-10（IL-10）、肿瘤坏死因子-α（TNF-α）、丙二醛（MDA）、超氧化物歧化酶（SOD）和诱导型一氧化氮合酶（iNOS）。与模型组比较，各剂量Ento-B均能降低UC大鼠CMDI（p < 0.05）、HS（p < 0.05或p < 0.01）评分，显著提高UC大鼠血清中IL-4、IL-10、SOD的表达（p < 0.01），降低血清中TNF-α、MDA、iNOS水平，显著改善UC大鼠结肠病变程度。Ento-B可能在UC大鼠溃疡性结肠炎的治疗中发挥重要作用。其机制可能与增加IL-4、IL-10、SOD的表达，降低TNF-α、MDA、iNOS的表达有关。
To study the Periplaneta americana L. extract Ento-B on the treatment of chronic ulcerative colitis induced by 2,4-dinitrochlorobenzene and acetic acid in rats and to explore its primary mechanism of action. Using 2,4-dinitrochlorobenzene combined with acetic acid to induce chronic ulcerative colitis (chronic UC) in rats. The sulfasalazine (400 mg/kg) and Ento-B (200 mg/kg, 100 mg/kg,50 mg/kg) were given by intragastric administration and the effect was evaluated according to the disease activity index (DAI) score, colon mucosal injury index (CMDI) score, histopathological score (HS) and the serum levels of Interleukin-4(IL-4), Interleukin-10(IL-10), Tumor necrosis factor-α(TNF-α), Malondialdehyde(MDA), Superoxide dismutase(SOD) and Inducible nitric oxide synthase(iNOS.) Compared with the model group, all doses of Ento-B could reduce the score of CMDI (p < 0.05), HS(p < 0.05 or p < 0.01), significantly increased the expression of IL-4, IL-10, SOD (p < 0.01) and decreased the levels of TNF-α, MDA, iNOS in serum of UC rats, significantly improving the degree of colon lesionsin UC rats. Ento-B may play an important role in the treatment of ulcerative colitis induced byUC rats. The mechanism may be related to the increased expression of IL-4, IL-10, SOD and reduced expression of TNF-α, MDA, iNOS.