RNA-seq between asexual archeospores and meiosis-related conchospores in Neopyropia yezoensis using Smart-seq2
RNA-seq between asexual archeospores and meiosis-related conchospores in Neopyropia yezoensis using Smart-seq2
复制标题
使用 Smart-seq2 对条斑新孢子的无性原孢子和减数分裂相关的壳孢子进行 RNA-seq
DOI：
10.1111/jpy.13197
复制
发表时间：
2021-08-04
影响因子：
2.9
通讯作者：
Wang, Guangce
中科院分区：
文献类型：
--
作者：
He, Bangxiang;Gu, Wenhui;Wang, Guangce
在条斑新孢子（海洋大型藻类的潜在模型系统）的生命周期中，无性原孢子和减数分裂相关的壳孢子都发育成菌体（配子体）。为了了解大型藻类中这种特殊的生命现象，我们挑选了两种孢子（每个样本10-30个细胞）并使用Smart-seq2进行RNA-seq。对比分析表明，古孢子中光捕获和碳固定相关的差异表达基因（DEG）表达上调，表明古孢子处于快速营养生长状态。在壳孢子中，蛋白质合成和降解，特别是分子伴侣，相关DEGs被上调，表明壳孢子中可能正在发生复杂的生命活动。壳孢子中表达了68个与DNA复制和修复相关的基因，表明壳孢子中可能发生活跃的DNA复制。此外，我们发现一种壳孢子仅在二倍体阶段（壳丝体、孢子囊丝）特异性表达DEG（py04595：DNA解旋酶），而三种原孢子仅在单倍体阶段（菌体）特异性表达DEG。这些分子水平结果表明，壳孢子更接近二倍体，可能是条斑鱼减数分裂母细胞。此外，我们发现可能与配子体从孢子体转变有关的结状同源盒基因（PyKNOX）仅在孢子体产生中表达，而在壳孢子、原孢子和菌体中不表达，表明条斑鱼配子体的形态发生可能需要PyKNOX的失活。
In the life cycle of Neopyropia yezoensis, a potential model system for marine macroalgae, both asexual archeospores and meiosis-related conchospores develop into thalli (gametophyte). To understand this special life phenomenon in macroalgae, we picked out the two kinds of spores (10-30 cells in each sample) and conducted RNA-seq using Smart-seq2. Comparative analysis showed that light capture and carbon fixation associated differentially expressed genes (DEGs) were upregulated in archeospores, thus indicating that archeospores are in a state of rapid vegetative growth. In conchospores, protein synthesis and degradation, especially molecular chaperone, associated DEGs were up-regulated, indicating that complex life activities might be occurring in conchospores. There were 68 genes related to DNA replication and repair expressed in conchospores, showing that active DNA replication might occur in conchospores. Moreover, we found that one conchospore specifically expressed DEG (py04595: DNA helicase) only in diploid stages (conchocelis, sporangial filament) and three archeospores specifically expressed DEGs only in haploid stages (thalli). These molecular level results indicated that conchospores were closer to diploid, and might be the meiotic mother cells of N. yezoensis. In addition, we found that the knotted-like homeobox gene (PyKNOX), which might relate to the transition of gametophyte from sporophyte, was only expressed in sporophyte generation but not expressed in conchospores, archeospores and thalli, indicating the morphogenesis of gametophyte sin N. yezoensis might require the inactivation of PyKNOX.