Up-regulation of antioxidative proteins TRX1, TXNL1 and TXNRD1 in the cortex of PTZ kindling seizure model mice
Up-regulation of antioxidative proteins TRX1, TXNL1 and TXNRD1 in the cortex of PTZ kindling seizure model mice
复制标题
PTZ点燃癫痫模型小鼠皮层抗氧化蛋白TRX1、TXNL1和TXNRD1的上调
DOI：
10.1371/journal.pone.0210670
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发表时间：
2019
期刊：
影响因子：
3.7
通讯作者：
Zhang Zhe
中科院分区：
文献类型：
--
作者：
Yu Jia Tian;Liu Ye;Dong Ping;Cheng Run En;Ke Shao Xi;Chen Kai Qin;Wang Jing Jing;Shen Zhong Shan;Tang Qiong Yao;Zhang Zhe
氧化应激被认为是癫痫所致脑损伤的发病机制之一。减少氧化应激可以改善癫痫发作时的脑损伤。然而，重要的抗氧化酶如硫氧还蛋白-1 （TRX1）、硫氧还蛋白样1蛋白（TXNL1）和硫氧还蛋白还原酶1 （TXNRD1）在癫痫发作期间的表达水平尚未研究。在本研究中，我们检测了PTZ诱发癫痫模型小鼠不同脑区TRX1、TXNL1和TXNRD1蛋白和mRNA的表达水平。我们发现急性和慢性癫痫模型小鼠皮层中TRX1、TXNL1和TXNRD1蛋白表达水平同时上调2- 3倍。但这些酶在癫痫模型小鼠海马、小脑和间脑中没有统一的表达模式变化。这些酶的mRNA表达在癫痫小鼠皮层中也有较小程度的上调。这些数据表明，抗氧化酶可能在癫痫发作期间对皮层的氧化应激提供保护作用。
Oxidative stress has been considered as one of pathogenesis of brain damage led by epilepsy. Reducing oxidative stress can ameliorate brain damage during seizures. However, expression levels of important antioxidative enzymes such as thioredoxin-1 (TRX1), thioredoxin-like 1 protein (TXNL1) and thioredoxin reductase 1 (TXNRD1) during seizures have not been investigated. In this study, we examined protein and mRNA expression levels of TRX1, TXNL1 and TXNRD1 in different brain regions in PTZ induced seizure model mice. We found that protein expression levels of TRX1, TXNL1 and TXNRD1 are simultaneously up-regulated by 2- or 3-fold in the cortex of both acute and chronic seizure model mice. But there is no unified expression pattern change of these enzymes in the hippocampus, cerebellum and diencephalon in the seizure model mice. Less extent up-regulation of mRNA expression of these enzymes were also observed in the cortex of seizure mice. These data suggest that antioxidative enzymes may provide a protective effect against oxidative stress in the cortex during seizures.