[Effect of 17beta-estradiol on Kv2.1 current and delayed rectifier potassium current in cultured rat hippocampal neurons].
[Effect of 17beta-estradiol on Kv2.1 current and delayed rectifier potassium current in cultured rat hippocampal neurons].
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发表时间：
2004-09
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通讯作者：
Wei Zhang-;Hong-wei Jin;Hai-xia Zhang;Shao-feng Xu;Hai-bo Yu;Xiao-Liang Wang
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作者：
Wei Zhang-;Hong-wei Jin;Hai-xia Zhang;Shao-feng Xu;Hai-bo Yu;Xiao-Liang Wang
目的研究17 -雌二醇对培养大鼠海马神经元Kv2.1钾通道电流和延迟整流钾电流（IK）的影响。方法采用全细胞膜片钳技术观察17 -雌二醇对培养大鼠海马神经元Kv2.1通道电流和IK的影响。结果β -雌二醇对Kv2.1电流和IK有浓度依赖性的降低作用。17 -雌二醇阻断Kv2.1和IK的ic50分别为2.4和4.0微mol × L（-1）。17 -雌二醇（3微摩尔× 1(-1)）使Kv2.1电流的稳态激活和失活曲线明显向负电位偏移。但只产生IK稳态激活曲线向负电位移动，对IK稳态失活无影响。结论17 -雌二醇对海马Kv2.1和IK的抑制作用相近。17 -雌二醇对IK电流的抑制作用可能部分通过阻断Kv2.1电流实现。
AIM To study the effects of 17beta-estradiol on Kv2.1 potassium channel current and delayed rectifier potassium current (IK) in cultured rat hippocampal neurons. METHODS The effects of 17beta-estradiol on Kv2.1 channel current and IK in cultured rat hippocampal neurons were observed using the whole cell patch clamp techniques. RESULTS 17beta-Estradiol was shown to reduce the amplitude of Kv2.1 current and IK in concentration-dependent manners. The IC50s of 17beta-estradiol blocking Kv2.1 and IK were 2.4 and 4.0 micromol x L(-1), respectively. 17beta-Estradiol (3 micromol x l(-1)) significantly shifted the steady-state activation and inactivation curves of Kv2.1 current to negative potentials. However, it only produced the shift of the steady-state activation curve of IK to the negative potential without effect on the steady-state inactivation of IK. CONCLUSION 17beta-Estradiol inhibits Kv2.1 and IK of hippocampus at similar level. The inhibition of 17beta-estradiol on IK current may be partially via blocking Kv2.1 current.