An insight into the salivary transcriptome and proteome of the adult female mosquito Culex pipiens quinquefasciatus
An insight into the salivary transcriptome and proteome of the adult female mosquito Culex pipiens quinquefasciatus
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DOI：
10.1016/j.ibmb.2004.02.008
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发表时间：
2004-06-01
影响因子：
3.8
通讯作者：
Valenzuela, JG
中科院分区：
文献类型：
--
作者：
Ribeiro, JMC;Charlab, R;Valenzuela, JG
为了深入了解成年雌性致倦库蚊的唾液转录组和蛋白质组（sialome），对cDNA文库进行随机测序，并获得所选蛋白质和肽的氨基末端信息。进一步分析了编码分泌蛋白的cDNA序列簇。转录组揭示了先前在其他吸血昆虫的唾液腺中报道的已知家族的几种蛋白质的编码信息，以及免疫相关产物，如C型凝集素，gambicin和prophenol氧化酶级联的成员。此外，还发现了几种编码低复杂性蛋白质的转录本，其中一些明显编码粘蛋白。发现了许多新的转录物，包括以前在蟹和虾中描述但在昆虫中没有描述的新的内切核酸酶;以前在蚊子唾液腺中没有描述但在毒腺和沙蝇和黑蝇的唾液腺中发现的透明质酸酶;几种富含半胱氨酸的肽，其可能具有抗凝血功能，包括类似于以前描述的抗蛋白酶的线虫家族的肽;和一个全新的富含半胱氨酸和色氨酸的蛋白家族（CWRC家族），其中描述了12个全长序列。还描述了另外14种新的蛋白质和肽，其功能和/或家族关系未知。总共描述了54种编码全长蛋白质的转录本。通过在SDS-PAGE/Edman降解实验中发现相应的氨基末端序列，证实了其中几种被翻译成蛋白质。所有表格和序列的电子版本均可在http://www.nebi.nlm.nih.gov/projects/Mosquito/C_quinquefasciatus_sialome上找到。爱思唯尔有限公司出版
To obtain an insight into the salivary transcriptome and proteome (sialome) of the adult female mosquito Culex quinquefasciatus, a cDNA library was randomly sequenced, and aminoterminal information for selected proteins and peptides was obtained. cDNA sequence clusters coding for secreted proteins were further analyzed. The transcriptome revealed messages coding for several proteins of known families previously reported in the salivary glands of other blood-feeding insects as well as immune-related products such as C-type lectin, gambicin, and members of the prophenol oxidase cascade. Additionally, several transcripts coding for low-complexity proteins were found, some clearly coding for mucins. Many novel transcripts were found, including a novel endonuclease previously described in crabs and shrimps but not in insects; a hyaluronidase, not described before in mosquito salivary glands but found in venom glands and in salivary glands of sand flies and black flies; several cysteine-rich peptides with possible anticlotting function, including one similar to a previously described nematode family of anti-proteases; and a completely novel family of cysteine- and tryptophane-rich proteins (CWRC family) for which 12 full-length sequences are described. Also described are 14 additional novel proteins and peptides whose function and/or family affiliation are unknown. In total, 54 transcripts coding for full-length proteins are described. That several of these are translated into proteins was confirmed by finding the corresponding aminoterminal sequences in the SDS-PAGE/Edman degradation experiments. Electronic versions of all tables and sequences can be found at http://www.nebi.nlm.nih.gov/projects/Mosquito/C_quinquefasciatus_sialome. Published by Elsevier Ltd.