Genome-wide identification of hexokinase gene family in Brassica napus: structure, phylogenetic analysis, expression, and functional characterization
Genome-wide identification of hexokinase gene family in Brassica napus: structure, phylogenetic analysis, expression, and functional characterization
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DOI：
10.1007/s00425-018-2888-8
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发表时间：
2018-04
期刊：
影响因子：
4.3
通讯作者：
Jingxue Wang;Xiaomin Wang;Siyu Geng;S. Singh;Yaohui Wang;Sitakanta Pattanaik;Ling Yuan
中科院分区：
文献类型：
--
作者：
Jingxue Wang;Xiaomin Wang;Siyu Geng;S. Singh;Yaohui Wang;Sitakanta Pattanaik;Ling Yuan
主要结论油菜己糖激酶基因家族的全基因组鉴定、表达分析和功能研究表明，该基因家族在植物生长发育中起着重要作用，在植物中，双功能己糖激酶（HXKs）多基因家族在影响生长发育的糖代谢和糖传感中起着重要作用。甘蓝型油菜（Brassica napusL.）是一种重要的油料作物，然而，人们对它知之甚少。油菜HXK基因家族。我们在B中鉴定了19个推定的HXKs。油菜基因组拉帕湾oleracea，B. napus对BnHXK基因的贡献几乎相等。系统发育分析将19 BnHXK分为4个类群。BnHXKs的外显子-内含子结构与拟南芥和水稻HXKs的外显子-内含子结构高度相似。组III和IVBnHXK在根中高度表达，而组I成员优先在叶中表达。对不同发育阶段种子转录组的分析表明，大多数I组和IVHXKs在授粉后2周（2 WAP）高度表达，而III组只有4 WAP。油菜品种在真菌感染后，表明可能参与防御反应。我们通过花粉介导的基因转化为BnHXK 9油菜产生了RNAi株系，BnHXK 9是相对较少表征的IV组成员。与野生型相比，BnHXK 9-RNAi株系的幼苗表现出延迟的生长。BnHXK 9基因的RNAi植株植株矮小，叶片卷曲，表明BnHXK 9基因参与了植物的发育。总之，我们的研究结果提供了一个重要作物中BnHXK基因家族的全面说明，并为进一步阐明其在糖代谢和传感以及植物生长发育中的作用奠定了基础。
Main conclusionGenome-wide identification, expression analysis, and functional characterization of previously uncharacterized hexokinase family of oil crop,Brassica napus, underscore the importance of this gene family in plant growth and development.In plants, the multi-gene family of dual-function hexokinases (HXKs) plays important roles in sugar metabolism and sensing that affect growth and development. Rapeseed (Brassica napusL.) is an important oil crop; however, little is known about theB. napus HXKgene family. We identified 19 putative HXKs inB. napusgenome.B. rapaandB. oleracea, the two diploid progenitors ofB. napus, contributed almost equally to theBnHXKgenes. Phylogenetic analysis divided the 19BnHXKsinto four groups. The exon–intron structures ofBnHXKsshare high similarity to those ofHXKsinArabidopsisand rice. The group III and IVBnHXKsare highly expressed in roots, whereas group I members preferentially express in leaves. Analysis of seed transcriptomes at different developmental stages showed that most of group I and IVHXKsare highly expressed 2-weeks after pollination (2WAP), compared to 4WAP for group III.BnHKXsare differentially expressed in susceptible and tolerantB. napuscultivars after fungal infection, suggesting the possible involvement in defense response. We generated rapeseed RNAi lines forBnHXK9, a member of relatively less characterized group IV, by pollen-mediated gene transformation. The seedlings ofBnHXK9-RNAi lines showed delayed growth compared to the wild type. The RNAi plants were dwarf with curly leaves, suggesting the involvement ofBnHXK9in plant development. Collectively, our findings provides a comprehensive account ofBnHXKgene family in an important crop and a starting point for further elucidation of their roles in sugar metabolism and sensing, as well as plant growth and development.