Molecular identification of endophytic fungi from medicinal plant Huperzia serrata based on rDNA ITS analysis
Molecular identification of endophytic fungi from medicinal plant Huperzia serrata based on rDNA ITS analysis
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DOI：
10.1007/s11274-010-0480-x
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发表时间：
2011-03-01
影响因子：
4.1
通讯作者：
Gan, Bing Chun
中科院分区：
文献类型：
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作者：
Chen, Xu Yu;Qi, Yao Dong;Gan, Bing Chun
从蛇足石杉(Huperzia serrata (Thunb. ex Murray) Trevis)的茎、叶和根中分离得到52株具有不同菌落形态的内生真菌。采自中国南部海南省霸王岭保护区。它们的鉴定主要基于rDNA ITS序列和系统发育分析。结果显示，所有菌株均属于伞菌纲（92.31%）、丝菌纲（3.85%）、盘菌纲（1.92%）和伞形菌纲（1.92%）4个纲。在属水平上鉴定出 47 个菌株，包括 Glomerella (Colletotrichum)、Hypocrea (Trichoderma)、Pleurostoma、Chaetomium、Coniochaeta (Lecythophora)、Daldinia、Xylaria、Hypoxylon、Nodulisporium、Cazia 和 Phellinus。至于其他5个菌株，其中3个在目水平上被鉴定出，2个在科水平上被鉴定出，这表明锯缘齿蠹中存在着丰富的真菌类群多样性。分离菌株大部分属于炭疽菌属和Hypoxylon属，其中炭疽菌属及其无性炭疽菌属21株（占分离菌株总数的42.3%），Hypoxylon属10株（占分离菌株总数的19.2%）。首次报道从 H. serrata 中分离出 Pleurostoma、Chaetomium、Coniochaeta (Lecythophora)、Daldinia、Xylaria、Hypoxylon、Nodulisporium、Cazia 和 Phellinus 等内生真菌。
Fifty-two endophytic fungi strains with different colony morphologies were isolated from stems, leaves and roots of Huperzia serrata (Thunb. ex Murray) Trevis. collected from Bawangling Reserve of Hainan Province in southern China. They were identified mainly based on rDNA ITS sequences and phylogenetic analysis. The results showed that all strains belonged to four classes, i.e. Sordariomycetes (92.31%), Dothideomycetes (3.85%), Pezizomycetes (1.92%) and Agaricomycetes (1.92%). Forty-seven strains were identified at the genus level, including Glomerella (Colletotrichum), Hypocrea (Trichoderma), Pleurostoma, Chaetomium, Coniochaeta (Lecythophora), Daldinia, Xylaria, Hypoxylon, Nodulisporium, Cazia and Phellinus. As to the other five strains, three were identified at the order level and two at the family level, indicating that a great diversity of fungi taxa exists in H. serrata. Most isolated strains belonged to the genus of Glomerella (Colletotrichum) and Hypoxylon, twenty-one from Glomerella and its anamorph Colletotrichum (42.3% of total isolated strains) and ten from Hypoxylon (19.2% of total isolated strains). Pleurostoma, Chaetomium, Coniochaeta (Lecythophora), Daldinia, Xylaria, Hypoxylon, Nodulisporium, Cazia and Phellinus were reported as endophytic fungi isolated from H. serrata for the first time.