A method of endotracheal intubation and pulmonary functional assessment for repeated studies in mice
A method of endotracheal intubation and pulmonary functional assessment for repeated studies in mice
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DOI：
10.1152/jappl.1999.87.6.2362
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发表时间：
1999-12-01
影响因子：
3.3
通讯作者：
Mitzner, W
中科院分区：
文献类型：
--
作者：
Brown, RH;Walters, DM;Mitzner, W
成功插管小鼠气管和控制其通气的能力对于需要从麻醉中恢复和重复肺功能测量或其他评估（如使用放射成像（如计算机断层扫描或磁共振成像））的纵向研究是重要的。我们描述了一种快速和重复插管小鼠的方法，随后在基线和激动剂刺激后测量肺功能。我们描述了一个简单构造的金属刀片用作喉镜，以方便口咽部暴露，颈部的透照，以方便通过口咽部观察气管，随时可用的聚乙烯管插管气管，简单的电磁呼吸机维持生理通气，评估呼吸阻力和顺应性。通过针向颈静脉短暂注入乙酰胆碱，评估气道平滑肌的反应性。
The ability to successfully intubate the trachea of mice and control their ventilation is important for longitudinal studies requiring recovery from anesthesia and repeated pulmonary function measurements or other evaluations, such as the use of radiological imaging (e.g., computed tomography or magnetic resonance imaging). We describe a method for rapid and repeated intubation of mice, with subsequent pulmonary function measurements at baseline and after an agonist challenge. We describe a simply constructed metal blade used as a laryngoscope to facilitate oropharyngeal exposure, transillumination of the neck to facilitate visualization of the trachea through the oropharynx, readily available polyethylene tubing to intubate the trachea, and a simple solenoid ventilator to maintain physiological ventilation and assess respiratory resistance and compliance Brief infusions of acetylcholine through a needle into the jugular vein are used to assess the responsiveness of the airway smooth muscle.