SR PROTEINS - A CONSERVED FAMILY OF PRE-MESSENGER-RNA SPLICING FACTORS
SR PROTEINS - A CONSERVED FAMILY OF PRE-MESSENGER-RNA SPLICING FACTORS
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DOI：
10.1101/gad.6.5.837
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发表时间：
1992-05-01
影响因子：
10.5
通讯作者：
ROTH, MB
中科院分区：
文献类型：
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作者：
ZAHLER, AM;LANE, WS;ROTH, MB
我们证明，来自小牛胸腺的四种不同蛋白质能够使用几种不同的前 mRNA 底物恢复相同剪接缺陷提取物中的剪接。这些蛋白质是由单克隆抗体识别的保守蛋白质家族的成员，该单克隆抗体与 RNA 聚合酶 II 转录的活性位点结合。我们通过两次盐沉淀纯化了该核磷蛋白家族，使其具有明显的同质性。该家族因其富含丝氨酸和精氨酸的羧基末端结构域而被称为 SR 蛋白，由至少五种不同的蛋白组成，分子量分别为 20、30、40、55 和 75 kD。微测序显示它们相关但不相同。在四个家族成员中观察到包含 RNA 识别基序的重复蛋白质序列。我们讨论了这组高度保守、功能相关的蛋白质在前 mRNA 剪接中的潜在作用。
We demonstrate that four different proteins from calf thymus are able to restore splicing in the same splicing-deficient extract using several different pre-mRNA substrates. These proteins are members of a conserved family of proteins recognized by a monoclonal antibody that binds to active sites of RNA polymerase II transcription. We purified this family of nuclear phosphoproteins to apparent homogeneity by two salt precipitations. The family, called SR proteins for their serine- and arginine-rich carboxy-terminal domains, consists of at least five different proteins with molecular masses of 20, 30, 40, 55, and 75 kD. Microsequencing revealed that they are related but not identical. In four of the family members a repeated protein sequence that encompasses an RNA recognition motif was observed. We discuss the potential role of this highly conserved, functionally related set of proteins in pre-mRNA splicing.