Brain metabolite composition during early human brain development as measured by quantitative in vivo 1H magnetic resonance spectroscopy
Brain metabolite composition during early human brain development as measured by quantitative in vivo 1H magnetic resonance spectroscopy
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DOI：
10.1002/mrm.10304
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发表时间：
2002-12-01
影响因子：
3.3
通讯作者：
Hüppi, PS
中科院分区：
文献类型：
--
作者：
Kreis, R;Hofmann, L;Hüppi, PS
在人类婴儿中，可以通过H-1磁共振波谱（MRS）无创地研究大脑的生化成熟。脑组织内容物的详细时间进程仅为最丰富的代谢物所知，早产是否影响大脑的生化成熟存在争议。因此，在早产儿中观察妊娠的最后三个月，并将其出生时和预期足月时的脑代谢物含量与足月儿进行比较。成功的定量短TE H-1 MRS在三个大脑的位置在21个婴儿在28个会话（胎龄32-43周）。采用线性组合模型拟合分析光谱，大大扩展了可观察代谢物的范围，包括乙酸盐、丙氨酸、天冬氨酸盐、胆碱、肌酸、γ-氨基丁酸盐、葡萄糖、谷氨酰胺、谷氨酸盐、谷胱甘肽、甘氨酸、乳酸盐、肌醇、大分子贡献、N-乙酰天冬氨酸盐、N-乙酰乙酰基谷氨酸盐、邻磷酸乙醇胺、鲨肌醇、牛磺酸和苏氨酸。年龄和位置的显着影响，发现许多代谢产物，包括以前观察到的神经元成熟反映了N-乙酰天冬氨酸的增加。足月早产儿的绝对脑代谢物含量与足月婴儿的绝对脑代谢物含量没有显著差异，表明早产并不影响研究人群（不包括极低出生体重的婴儿）的生化脑成熟。
Biochemical maturation of the brain can be studied noninvasively by H-1 magnetic resonance spectroscopy (MRS) in human infants. Detailed time courses of cerebral tissue contents are known for the most abundant metabolites only, and whether or not premature birth affects biochemical maturation of the brain is disputed. Hence, the last trimester of gestation was observed in infants born prematurely, and their cerebral metabolite contents at birth and at expected term were compared with those of fullterm infants. Successful quantitative short-TE H-1 MRS was performed in three cerebral locations in 21 infants in 28 sessions (gestational age 32-43 weeks). The spectra were analyzed with linear combination model fitting, considerably extending the range of observable metabolites to include acetate, alanine, aspartate, cholines, creatines, gamma-aminobutyrate, glucose, glutamine, glutamate, glutathione, glycine, lactate, myo-inositol, macromolecular contributions, N-acetylaspartate, N-acetylaspartylglutamate, o-phosphoethanolamine, scyllo-inositol, taurine, and threonine. Significant effects of age and location were found for many metabolites, including the previously observed neuronal maturation reflected by an increase in N-acetylaspartate. Absolute brain metabolite content in premature infants at term was not considerably different from that in fullterm infants, indicating that prematurity did not affect biochemical brain maturation substantially in the studied population, which did not include infants of extremely low birthweight.