Type IV pili, transient bacterial aggregates, and virulence of enteropathogenic Escherichia coli
Type IV pili, transient bacterial aggregates, and virulence of enteropathogenic Escherichia coli
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DOI：
10.1126/science.280.5372.2114
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发表时间：
1998-06-26
期刊：
影响因子：
56.9
通讯作者：
Schoolnik, GK
中科院分区：
文献类型：
--
作者：
Bieber, D;Ramer, SW;Schoolnik, GK
肠致病性大肠杆菌的IV型菌毛形成丸是局部粘附和自聚集表型所必需的，通过分别灭活菌毛亚基和bfp操纵子转录激活因子的bfpA或bfpT（perA）编码，在志愿者中测试这些丸是否也是毒力所必需的。两种突变体引起的腹泻明显减少。bfpF核苷酸结合结构域的突变引起了毛发形成的增加，增强了局部粘附，并消除了自聚集表型的抽搐运动分散相。bfpF突变体定殖于人类肠道，但毒性降低约200倍。因此，BfpF是从细菌聚集体分散和完全毒力所必需的。
Type IV bundle-forming pill of enteropathogenic Escherichia coli are required for the localized adherence and autoaggregation phenotypes, Whether these pill are also required for virulence was tested in volunteers by inactivating bfpA or bfpT (perA) encoding, respectively, the pilus subunit and the bfp operon transcriptional activator. Both mutants caused significantly less diarrhea. Mutation of the bfpF nucleotide-binding domain Caused increased piliation, enhanced localized adherence, and abolished the twitching motility-dispersal phase of the autoaggregation phenotype. The bfpF mutant colonized the human intestine but was about 200-fold less virulent. Thus, BfpF is required for dispersal from the bacterial aggregate and for full virulence.