Imbalanced distribution of Plasmodium falciparum EBA-175 genotypes related to clinical status in children from Bakoumba, Gabon.
Imbalanced distribution of Plasmodium falciparum EBA-175 genotypes related to clinical status in children from Bakoumba, Gabon.
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加蓬巴库巴儿童中与临床状况相关的恶性疟原虫 EBA-175 基因型分布不平衡。
DOI：
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发表时间：
2006
期刊：
影响因子：
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通讯作者：
E. Mavoungou
中科院分区：
文献类型：
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作者：
F. Touré;Cyrille Bisseye;E. Mavoungou
目的
恶性疟原虫红细胞结合抗原175 kDa（EBA-175）是裂殖子侵入红细胞的主要配体之一。EBA-175是一种二态性抗原，但二态性在宿主寄生虫相互作用中的作用尚未完全了解。在这项研究中，我们试图确定EBA-175基因型的分布和其发病的影响。
方法
巢式聚合酶链反应用于确定无症状和有症状的加蓬儿童恶性疟原虫分离株的基因型。
结果
CAMP菌株（C段）和FCR-3菌株（F段）分别在13/50（26%）和19/50（38%）有症状的儿童中发现，分别在16/66（24%）和46/66（70%）无症状儿童中发现。C-/F-混合感染在有症状和无症状儿童中的患病率分别为18/50（36%）和4/66（6%）。
结论
这些结果表明，C-/F-混合感染与临床疟疾有关（χ 2，P <0.01），并可能具有重要的治疗意义。
OBJECTIVE
The erythrocyte binding antigen 175 kDa (EBA-175) of Plasmodium falciparum is one of the major ligands for red blood cell invasion by merozoites. EBA-175 is a dimorphic antigen but the role that dimorphism plays in host parasite interaction is not fully understood. In this study, we sought to determine the distribution of EBA-175 genotypes and its pathogenetic influence.
METHODS
The nested polymerase chain reaction was used to determine the genotypes of P. falciparum isolates from asymptomatic and symptomatic Gabonese children.
RESULTS
CAMP strains (C-segment) and FCR-3 strains (F-segment) were found in 13/50 (26%) and 19/50 (38%) symptomatic children, respectively and in 16/66 (24%) and 46/66 (70%) asymptomatic children, respectively. The prevalence of mixed C-/F- infection was 18/50 (36%) and 4/66 (6%) in symptomatic and asymptomatic children, respectively.
CONCLUSIONS
These results show that mixed C-/F- infection is associated with clinical malaria (chi2, P <0.01) and may have important therapeutic implications.