Modelling the transport of environmental DNA through a porous substrate using continuous flow-through column experiments
Modelling the transport of environmental DNA through a porous substrate using continuous flow-through column experiments
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使用连续流通柱实验模拟环境 DNA 通过多孔基质的运输
DOI：
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发表时间：
2016
影响因子：
3.9
通讯作者：
D. Bolster
中科院分区：
文献类型：
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作者：
Arial J. Shogren;J. Tank;Elizabeth A. Andruszkiewicz;Brett P. Olds;C. Jerde;D. Bolster
检测水样中的环境DNA(EDNA)是确定稀有水生物种存在的有力工具。然而，关于流水中的生物和物理条件如何影响埃德娜，仍有许多悬而未决的问题。受到人们在溪流/河流底栖动物中可能发现的东西的启发，我们在填充了多孔底物的连续流动柱中进行了实验，以探索EDNA的传输，并询问底物类型和定植生物膜的存在是否对EDNA的保留起着重要作用。为了解释我们的数据，并出于建模的目的，我们首先假设Edna可以被视为经典的示踪剂。将我们的实验数据与传统的输运模型进行比较，我们发现Edna的行为异常，表现出一种多相、多分散的物质的特征，具有颗粒状的行为，可以被基质过滤。根据质量平衡计算，柱上的悬浮EDNA颗粒很快被冲洗掉，而相当数量的颗粒从未通过，并被保留在柱内。悬浮的埃德娜是通过柱子出口的，无论生物膜的定植情况如何。我们的结果表明，EDNA的不同粒度导致了随机的滞留、释放和传输，这可能会影响生物系统中EDNA的解释。
Detecting environmental DNA (eDNA) in water samples is a powerful tool in determining the presence of rare aquatic species. However, many open questions remain as to how biological and physical conditions in flowing waters influence eDNA. Motivated by what one might find in a stream/river benthos we conducted experiments in continuous flow columns packed with porous substrates to explore eDNA transport and ask whether substrate type and the presence of colonized biofilms plays an important role for eDNA retention. To interpret our data, and for modelling purposes, we began with the assumption that eDNA could be treated as a classical tracer. Comparing our experimental data with traditional transport models, we found that eDNA behaves anomalously, displaying characteristics of a heterogeneous, polydisperse substance with particle-like behaviour that can be filtered by the substrate. Columns were quickly flushed of suspended eDNA particles while a significant amount of particles never made it through and were retained in the column, as calculated from a mass balance. Suspended eDNA was exported through the column, regardless of biofilm colonization. Our results indicate that the variable particle size of eDNA results in stochastic retention, release and transport, which may influence the interpretation eDNA detection in biological systems.