HIGH-LEVEL OF INTERLEUKIN-10 PRODUCTION BY THE ACTIVATED T-CELL POPULATION WITHIN THE RHEUMATOID SYNOVIAL-MEMBRANE
HIGH-LEVEL OF INTERLEUKIN-10 PRODUCTION BY THE ACTIVATED T-CELL POPULATION WITHIN THE RHEUMATOID SYNOVIAL-MEMBRANE
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DOI：
10.1002/art.1780380710
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发表时间：
1995-07-01
影响因子：
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通讯作者：
FELDMANN, M
中科院分区：
文献类型：
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作者：
COHEN, SBA;KATSIKIS, PD;FELDMANN, M
目的探讨类风湿关节炎（RA）患者滑膜中活化T细胞的细胞因子特性，方法采用白细胞介素-2（IL-2）筛选2例RA患者滑膜中活化T细胞，并进行非特异性克隆。将这些克隆的细胞因子产生谱与来源于外周血单核细胞（PBM）的克隆的细胞因子产生谱进行比较，方法是用固定的抗-CD 3（以10 μ g/ml包被）或佛波醇-12-肉豆蔻酸酯-13-乙酸酯（10 ng/ml）加可溶性抗-CD 3（1 μ g/ml）刺激所有克隆24小时。干扰素采用酶联免疫吸附试验检测了区分Th 1和Th 2细胞并参与免疫调节的细胞因子（IFN γ）、IL-4和IL-10。结果，类风湿膜来源的克隆与外周来源的克隆产生的细胞因子存在差异，膜和PBM来源的克隆大多是IFN γ产生者，也就是说，Th 0或Th 1型，有高比例的IFN γ/高IL-10产生细胞来源于关节，而不是来自外周。在来自2名RA患者的滑膜的克隆中，100%和50%产生1-10 ng/ml IFN γ和>7 ng/ml IL-10，与之相比，
Objective, To characterize the cytokine profile of the activated T cell population derived from the synovial membrane of rheumatoid arthritis (RA) patients.Methods, Interleukin-% (IL-2) was used to select for in vivo-activated T cells from the synovial membrane of 2 patients with RA, and the cells were cloned nonspecifically. The cytokine production profile of these clones was compared with that of clones derived from peripheral blood monocytes (PBM) by stimulating all clones for 24 hours with immobilized anti-CD3 (coated at 10 mu g/ml) or phorbol-12-myristate-13-acetate (10 ng/ml) plus soluble anti-CD3 (1 mu g/ml). Interferon-gamma (IFN gamma), IL-4, and IL-10, the cytokines that discriminate between Th1 and Th2 cells and are involved in immunoregulation, were assayed by enzyme-linked immunosorbent assay.Results, There was a difference in the cytokines produced by the clones derived from the rheumatoid membranes compared with clones derived from the periphery, Clones derived from both membranes and PBM were mostly IFN gamma-producers, i.e., either a Th0 or a Th1 profile, There was a high proportion of IFN gamma/high IL-10-producing cells derived from the joint, but not from the periphery, Of clones derived from the synovial membrane of each of 2 RA patients, 100% and 50% produced both 1-10 ng/ml IFN gamma and >7 ng/ml IL-10, compared with